Fig 1: HNRNPA3 dimethylation and its correlation with inflammatory factors. (A) Greatly increased asymmetric arginine dimethylation of HNRNPA3 proteins in CP tissues. Protein asymmetric arginine dimethylation were quantitatively assessed by immunoprecipitation using antibodies targeting ADMA, followed by Western blot with the antibodies specifically recognizing HNRNPA3 (B). Relative HNRNPA3 dimethylation levels among four CP patients were compared with the control group containing four other cholangiocarcinoma patients. (C, Dand E) The positive correlation of HNRNPA3 asymmetric arginine dimethylation levels with the major inflammatory factors in serum of CP patients. Serum TGF-b, TNF-a and IL-6 levels in CP patients were analyzed via ELISA method. *P<0.05.
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