Fig 1: Rapamycin increases the mitophagy marker BNIP3 at short-time intervals within U87MG cells. (A) Representative BNIP3-stained mitochondrion (indicated by the black arrow) at 24 h during 10 nM rapamycin, continuous exposure. Graphs report: (B) the amount of total BNIP3 particles; (C) the number of BNIP3-positive mitochondria; (D) the ratio of mitochondrial/cytosolic BNIP3 particles at various time interval during (12–24 h) or following (from 1 d up to 14 d) rapamycin exposure. M = mitochondrion, AV = vacuoles. * p = 0.05 vs. Control; ** p = 0.05 vs. Control and 12 h; *** p = 0 05 vs. other groups. Scale bar = 0.3 µm.