Fig 2: Interactions between HuR and SOCS7. a Purification of SOCS7 immunocomplex (arrows). Proteins were separated on SDS-PAGE and stained by Coomassie Blue. b and c The interactions between HuR and SOCS7 in CAOV3 (b) and OVCAR3 (c) cell lysates. d Subcellular localizations of SOCS7 (green) and HuR (red). Nuclei were stained with DAPI (blue) for reference. Scale bar = 50 µm. e and f The mRNA (e) and protein (f) levels of HuR in CAOV3, OVCAR3, and COV318 cells transduced with indicated lentiviral vectors. g SOCS7 and HuR protein expression in CAOV3 and OVCAR3 cells transduced with indicated lentiviral vectors and treated with 10 µM proteasome inhibitor (MG132) or DMSO. h SOCS7/HuR protein expression and the ubiquitination of HuR in CAOV3 cells

Fig 3: SOCS7 regulates HGSOC cell growth both in vitro and in vivo. a The mRNA and protein levels of SOCS7 in various HGSOC cell lines and human ovarian epithelial cell line IOSE80. b Association between SOCS7 expression and cell cycle, based on GSEA analysis. c-e SOCS7 overexpression and knockdown in HGSOC cells transduced with indicated lentiviral vectors. f–h Viability and i-n cell cycle of HGSOC cells transduced with indicated lentiviral vectors. o-r CAOV3 cells transduced with pLVX-Puro-SOCS7 or blank pLVX-Puro were injected into nude mice, and SOCS7 expression levels (o), tumor volume (p), weight (q), and the immunofluorescence staining for Ki67 (r) were determined. Scale bar = 50 µm. *P < 0.05, **P < 0.01, and ***P < 0.001, in related with IOSE80, shNC or Vector

Fig 4: The relationship among the expression levels of SOCS7/HuR/FOXM1 in the tumor tissues of HGSOC patient and their correlations with survival rate of HGSOC patients. a IHC staining analysis of SOCS7, HuR, and FOXM1 in the tumor tissues of HGSOC patients in cohort 2. Scale bar = 100 µm. b-d Spearman's correlation scatter plots for HuR-SOCS7 (b), HuR-FOXM1 (c) and FOXM1-SOCS7 (d) of HGSOC patients in cohort 2. e and f The overall survival of HGSOC patients with different protein levels of HuR (e) or FOXM1 (f) in cohort 2. g The overall survival of HGSOC patients with different protein levels of SOCS7, HuR, and FOXM1 in cohort 2. h Schematic model of the regulation of HGSOC tumor growth by SOCS7/HuR/FOXM1

Fig 5: FOXM1 is involved in the regulation of HGSOC cell viability and cell cycle progression by SOCS7. a FOXM1 expression in CAOV3 cells transduced with indicated lentiviral vectors. b Viability, c and d cell cycle distribution of CAOV3 cells transduced with indicated lentiviral vectors. e and f FOXM1 mRNA and protein expression in CAOV3 cells transduced with indicated lentiviral vectors. *P < 0.05, **P < 0.01 and ***P < 0.001, in related with Vector. ##P < 0.01, and ###P < 0.001, in related with SOCS7