Fig 1: SMAD and TEAD stimulate 6PGL transcription in a YAP-dependent manner.A Predicting 6PGL promoter binding YAP-associated transcription factors by JASPAR database. B 6PGL mRNA was measured in A549 and H1299 cells with indicated plasmids overexpressed. C The enrichments of YAP at indicated regions of the 6PGL promoter were calculated as the percentage of Input chromosomal DNA via ChIP in A549 cells. Anti-IgG and anti-TFCP2 were used as parallel control. D, E Luciferase activity was analyzed in A549 cells co-expressing indicated 6PGL promoter–reporter with or without overexpressing (D) or knocking out YAP (E). F, G Co-occupancies of YAP and SMAD2 (F) and YAP and TEAD4 (G) were measured by ChIP and Re-ChIP experiments in A549 cells using indicated antibodies. H Nuclear extracts from A549 cells were incubated with indicated 6PGL promoter probes with or without the presence of indicated antibodies. The DNA–protein interactions were measured using EMSA. The data are shown as the mean ± SD from three biological replicates (including IB). Data in B–E were analyzed using a one-way ANOVA test. **P < 0.01, *P < 0.05, NS nonsignificant.