Anti-CD4 antibody [EPR19514] ab183685 from Abcam

Product Specs
ItemAnti-CD4 antibody [EPR19514]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab183685
Research AreaImmuno-oncology
Gene NameCD4
ReactivityMouse
TargetCD4
ClonalityMonoclonal
NCBI Gene AliasesL3T4, Ly-4
ApplicationsWB, IHC-fr, IHC-p, IP
Antibody TypePrimary
HostRabbit
CloneEPR19514
IsotypeIgG
Conjugate/TagUnconjugated
Quantity10 UL, 40 UL, 100 UL

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Host genetic variation guides hepacivirus clearance, chronicity, and liver fibrosis in miceHepatologyJanuary 1, 2024Ariane J. Brown, John J. Won, Raphael Wolfisberg, Ulrik Fahnøe, Nicholas Catanzaro, Ande West, Fernando R. Moreira, Mariana Nogueira Batista, Martin T. Ferris, Colton L. Linnertz, et al.Trichrome (Richard-Allen Scientific). Dual Chromogenic Immunohistochemistry was accomplished using CD4 (ab183685, Abcam) and CD8 (14-0808-82, Invitrogen) antibodies on the Ventana Discovery platform (Roche). The slides

(2) Euphorbia factor L2 suppresses the generation of liver metastatic ascites in breast cancer via inhibiting NLRP3 inflammasome activationInt J Mol MedDecember 4, 2023Dongjing Jiang, Xun Liu, Rulan Tan, Ye Zhu, Li Zhang14708) were purchased from Cell Signaling Technology, Inc. Antibodies specifically against CD4 (cat. no. Ab183685) and CD8 (cat. no. Ab209775) were purchased from Abcam. Dulbecco's modified Eagle medium (DMEM) and

(3) Chronic metabolic stress drives developmental programs and loss of tissue functions in non-transformed liver that mirror tumor states and stratify survivalbioRxivDecember 1, 2023Constantine N. Tzouanas, Marc S. Sherman, Jessica E.S. Shay, Adam J. Rubin, Benjamin E. Mead, Tyler T. Dao, Titus Butzlaff, Miyeko D. Mana, Kellie E. Kolb, Chad Walesky, et al.1:400), anti-CXCR6 (Thermo Fisher, PA5-79117; 1:125), anti-TOX (Abcam, ab237009; 1:50), anti-CD4 (Abcam, ab183685; 1:500), and anti-CD8A (Abcam, ab217344; 1:500). Imaging was conducted on a confocal Nikon Ti2 microscope

(4) Cooperation of MLL1 and Jun in controlling H3K4me3 on enhancers in colorectal cancerGenome BiologyNovember 27, 2023Xiang Lin, Ji-Dong Chen, Chen-Yu Wang, Zhen Cai, Rui Zhan, Chen Yang, La-Ying Zhang, Lian-Yun Li, Yong Xiao, Ming-Kai Chen, et al.RRID:AB_2688010), JUN (CST, 9165, RRID:AB_2130165), CD3 (Abcam, ab16669, RRID:AB_443425), CD4 (Abcam, ab183685, RRID:AB_2686917), CD8 (Abcam, ab217344, RRID:AB_2890649), F4/80(CST, 70,076, RRID:AB_2799771), CD206

(5) Epidermal keratinocyte-specific STAT3 deficiency aggravated atopic dermatitis-like skin inflammation in mice through TSLP upregulationFrontiers in ImmunologyNovember 20, 2023Zhao-Yuan Wang, Yu-Xin Zheng, Fan Xu, Ying-Zhe Cui, Xue-Yan Chen, Si-Qi Chen, Bing-Xi Yan, Yuan Zhou, Min Zheng, Xiao-Yong ManBurlingame, CA, USA). For IF, slides were incubated with K14 (Abcam, ab181595, 1:500), CD4 (Abcam, ab183685, 1:500), CD8 (R&D, MAB116, 1:100), loricrin (Proteintech, 55439-1-AP, 1:100), filaggrin (Abcam, ab81468,

(6) Combination of verteporfin-photodynamic therapy with 5-aza-2’-deoxycytidine enhances the anti-tumour immune response in triple negative breast cancerFrontiers in ImmunologyNovember 7, 2023Shramana M. Banerjee, Pilar Acedo, Soha El Sheikh, Rania Harati, Amelia Meecham, Norman R. Williams, Gareth Gerard, Mohammed R. S. Keshtgar, Alexander J. MacRobert, Rifat Hamoudiinvasion, increase in immunological activity were also recorded. Immunohistochemistry, using anti-CD4 (ab183685, Abcam, 1:500 dilution) and anti-CD8 (ab209775, Abcam, 1:1000 dilution) antibodies, was employed to

(7) Hepatic macrophages play critical roles in the establishment and growth of hydatid cysts in the liver during Echinococcus granulosus sensu stricto infectionPLOS Neglected Tropical DiseasesNovember 6, 2023Hui Wang, Qian Yu, Mingkun Wang, Jiao Hou, Maolin Wang, Xuejiao Kang, Xinling Hou, Dewei Li, Zibigu Rousu, Tiemin Jiang, et al.anti-human/mouse α-SMA, 1:500, ab 124964; anti-mouse F4/80, 1:100, ab6640; anti-mouse CD4, 1:1000, ab183685 (all from Abcam, Cambridge, UK); and anti-mouse Collagen Type III Alpha 1 (Col3α1), 1:2000 (Cat: 2273,

(8) Topical Calcipotriol Plus Imiquimod Immunotherapy for Nonkeratinocyte Skin CancersJID InnovationsNovember 1, 2023Marjan Azin, Kenneth H. Ngo, Jennet Hojanazarova, Shadmehr Demehriantibody (catalog number ab11089, Abcam, Cambridge, United Kingdom) and anti-CD4 antibody (catalog number ab183685, Abcam) overnight at 4 °C. After 12 hours, the sections were washed with 0.1% Tween 20, blocked in blocking

(9) Adjuvant-dependent effects on the safety and efficacy of inactivated SARS-CoV-2 vaccines during heterologous infection by a SARS-related coronavirusResearch squareOctober 27, 2023Heise M, Dillard J, Taft-Benz S, Knight A, Anderson E, Pressey K, Parotti B, Martinez S, Diaz J, Sarkar S, et al.positively charged slides. Sequential tissue sections were labeled for antigens using anti-CD4 antibody (ab183685, Abcam), anti-C3 Complement antibody (55444, MP Biomedical), or Arginase-1 (93668S, Cell Signaling Technology)

(10) Propolis Has an Anticancer Effect on Early Stage Colorectal Cancer by Affecting Epithelial Differentiation and Gut Immunity in the Tumor MicroenvironmentNutrientsOctober 24, 2023Ming-Hung Shen, Chih-Yi Liu, Kang-Wei Chang, Ching-Long Lai, Shih-Chang Chang, Chi-Jung Huanganti-CD3 (clone 2GV6, prediluted; cat. no. 790-4341, Roche Diagnostics) for 2 h, anti-CD4 (1:200; cat. no. ab183685, Abcam, Cambridge, UK) for 2 h, or anti-FOXP3 (1:50; cat. no. ab 215206, Abcam) for 2 h. N-Histofine

(11) GPR4 Knockout Attenuates Intestinal Inflammation and Forestalls the Development of Colitis-Associated Colorectal Cancer in Murine ModelsCancers (Basel)October 13, 2023Mona A. Marie, Edward J. Sanderlin, Alexander P. Hoffman, Kylie D. Cashwell, Swati Satturwar, Heng Hong, Ying Sun, Li V. YangCambridge, MA, USA), anti-F4/80 (Cell Signaling Technology, #70076, Danvers, MA, USA), anti-CD4 (Abcam #ab183685, Waltham, MA, USA), anti-CD8 (Abcam, #ab203035, Waltham, MA, USA),anti-cleaved caspase-3 (Cell Signaling Technology

(12) Transcriptional immunogenomic analysis reveals distinct immunological clusters in paediatric nervous system tumoursGenome MedicineSeptember 7, 2023Arash Nabbi, Pengbo Beck, Alberto Delaidelli, Derek A. Oldridge, Sumedha Sudhaman, Kelsey Zhu, S. Y. Cindy Yang, David T. Mulder, Jeffrey P. Bruce, Joseph N. Paulson, et al.was performed using the Ventana Discovery platform. IHC was optimized and performed with CD4 (Abcam Ab183685), CD8 (Leica NCL-L-CD8-4B11) and CD19 (e-Bioscience 14–0194) with dilutions of 1:500, 1:100 and 1:500

(13) Immunobiology of a rationally-designed AAV2 capsid following intravitreal delivery in miceGene TherapySeptember 1, 2023Michael Whitehead, Andrew Sage, Tom Burgoyne, Andrew Osborne, Patrick Yu-Wai-Man, Keith R. Martinretrieval RBPMS,Rabbit,1:500,Abcam,ab152101,No GFAP,Rabbit,1:500,Dako,Z0334,No Iba1,Guinea pig,1:250,Synaptic systems,234 004,No CD4,Rabbit,1:250,Abcam,ab183685,Yes CD8,Rabbit,1:1000,Abcam,ab217344,Yes List of primary antibodies used in immunohistochemical

(14) Increased α-HB links colorectal cancer and diabetes by potentiating NF-κB signalingMolecular MetabolismSeptember 1, 2023Xinyue Lv, Peipei Ding, Luying Li, Ling Li, Danlei Zhou, Xiaochao Wang, Jianfeng Chen, Wei Zhang, Qi Wang, Tian Liao, et al.dilution for western blotting), HA (MBL, 1:100 working dilution for western blotting), CD4 (Abcam, ab183685, 1:1000 working dilution for IHC), and CD8 (Abcam, ab217344, 1:1000 working dilution for IHC) were purchased

(15) Histone deacetylase-mediated tumor microenvironment characteristics and synergistic immunotherapy in gastric cancerTheranosticsAugust 18, 2023Yilin Lin, Xiangxiang Jing, Zhihua Chen, Xiaoxian Pan, Duo Xu, Xiang Yu, Fengyun Zhong, Long Zhao, Changjiang Yang, Bo Wang, et al.tablets were sealed with anti-fluorescence quenching tablets. Antibodies against CD8α (ab237709), CD4 (ab183685), pan-CK (ab7753) and PD-L1 (ab213480) were purchased from Abcam. The slices were placed under a scanner

(16) Gene Immunotherapy of Colon Carcinoma with IL-2 and IL-12 Using Gene ElectrotransferInt J Mol SciAugust 17, 2023Tilen Komel, Masa Omerzel, Urska Kamensek, Katarina Znidar, Ursa Lampreht Tratar, Simona Kranjc Brezar, Klemen Dolinar, Sergej Pirkmajer, Gregor Sersa, Maja CemazarF4/80 (ab111101), 1/200 for rabbit anti-mouse MHC class II (ab180779), 1/100 for rabbit anti-mouse CD4 (ab183685), 1/1000 for rabbit anti-mouse CD8 (ab209775), 1/200 for rabbit anti-mouse CD31 (ab28364) (Abcam, Cambridge,

(17) Accumulation of Linoleic Acid by Altered Peroxisome Proliferator-Activated Receptor-α Signaling Is Associated with Age-Dependent Hepatocarcinogenesis in Ppara Transgenic MiceMetabolitesAugust 10, 2023Xiaoyang Zhu, Qing Liu, Andrew D. Patterson, Arun K. Sharma, Shantu G. Amin, Samuel M. Cohen, Frank J. Gonzalez, Jeffrey M. Petersthree times. After blocking and washing, slides were incubated with either an anti-CD4 antibody (Abcam, ab183685, Waltham, MA, USA), an anti-p21 antibody (Abcam, ab188224, Waltham, MA, USA), or an anti-CDKN2A/p16INK4a

(18) Dual Blockade of EP2 and EP4 Signaling is Required for Optimal Immune Activation and Anti-Tumor Activity Against Prostaglandin-Expressing TumorsCancer Research CommunicationsAugust 8, 2023Brian J. Francica, Anja Holtz, Justine M Lopez, David Freund, Austin Chen, Dingzhi Wang, David Powell, Franciele C Kipper, Dipak Panigrahy, Raymond N. DuBois, et al.Antigen,Clone,Catalog no.,Source CD8α,4SM15,13-0808-82,Thermo Fisher Scientific CD4,EPR19514,ab183685,Abcam F4/80,BM8,14-4801-82,Thermo Fisher Scientific Antibodies used for IHC of CT26 tumors

(19) Targeting of SLC25A22 boosts the immunotherapeutic response in KRAS-mutant colorectal cancerNature communicationsAugust 4, 2023Zhou Q, Peng Y, Ji F, Chen H, Kang W, Chan LS, Gou H, Lin Y, Huang P, Chen D, et al.peroxidase activity. Slides were incubated with primary antibodies overnight, including anti-CD4 (1:2000, ab183685, Abcam), anti-CD8 (1:2000, ab217344, Abcam), anti-S100A8 (1:800, 15792-1-AP, Proteintech), or anti-SLC25A22

(20) An immune-based tool platform for in vivo cell clearanceLife Science AllianceAugust 1, 2023Jieqiong Zhang, Tatsuya Tsukui, Xiumin Wu, Alyssa Brito, John Maxwell Trumble, Juan C Caraballo, Greg M Allen, José Zavala-Solorio, Chunlian Zhang, Jonathan Paw, et al.incubated with a primary antibody (OVA antibody; Millipore, AB1225, at 1:1500 dilution; Anti-CD4: Abcam, ab183685, Lot# GR3240246; Anti-CD8 antibody: Cell Signaling #98941) for overnight at 4°. After several rinses

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Anti-CD4 antibody [EPR19514] from Abcam

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Anti-CD4 antibody [EPR19514] from Abcam

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