Fig 1: Kaplan-Meier survival analyses of NPC1L1/ NPC2 combination in HCC patients. (A) Probability of survival of postoperative HCC patients: all low expression (n=169, mean=45.0 months), NPC1L1 low/NPC2 high or NPC1L1 high/NPC2 low expression (n=68, mean=61.7 months), all high expression (n=28, mean=61.1 months) (B) Probability of recurrence of postoperative HCC patients: all low expression (n=169, mean=34.2 months), NPC1L1 low/NPC2 high or NPC1L1 high/NPC2 low expression (n=68, mean=41.6 months), all high expression (n=28, mean=54.4 months)
Fig 2: Kaplan-Meier survival analyses of NPC1L1 and NPC2 in HCC patients. (A) Probability of survival of postoperative HCC patients: low expression of NPC1L1 (n=222, mean=53.5 months), high expression of NPC1L1 (n=43, mean=59.1 months). (B) Probability of recurrence of postoperative HCC patients: low expression of NPC1L1 (n=222, mean=35.9 months), high expression of NPC1L1 (n=43, mean=50.6 months). (C) Probability of survival of postoperative HCC patients: low expression of NPC2 (n=184, mean=45.7 months), high expression of NPC1L1 (n=81, mean=65.4 months). (D) Probability of recurrence of postoperative HCC patients: low expression of NPC2 (n=184, mean=34.8 months), high expression of NPC1L1 (n=81, mean=46.4 months).
Fig 3: NPC1L1 and NPC2 expression in peritumoral tissue and paired HCC tissues by Wetern-blot and Real-time Quantitative PCR. (A) NPC1L1 and NPC2 expression was detected by Western blot analyses in 10 paired HCC tissue (T) and peritumoral tissue (P). (B) Quantitation of proteins from Western blot analyses shows that both NPC1L1 and NPC2 expressions significantly reduced in HCC tissue (T) (p=0.0002 for NPC1L1, p=0.0489 for NPC2). The mRNA expression of NPC1L1 and NPC2 was detected by Real-time Quantitative PCR. Results clearly shows that mRNA expression levels also reduced in HCC tissue (T) compared to peritumoral tissue (P) for both NPC1L1 (C) and NPC2 (D).
Fig 4: NPC1L1 and NPC2 expression in peritumoral tissue and paired HCC tissues by immunohistochemistry. Expression features of NPC1L1 in (A) paired peritumoral tissue and (B) HCC tissue (×200). Expression of NPC2 in (C) paired peritumoral tissue and (D) HCC tissue (×200). Picture contains both HCC and peritumoral tissue for (E) NPC1L1 and (F) NPC2 (×100). (G) Scatter plot showing the mean staining intensity of paired peritumoral tissue (P) and HCC tumor tissue (T) (p<0.0001 for NPC1L1, p=0.0001 for NPC2).
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