Fig 1: KO of AFDN and SCRIB in MCF7 cells.a Individual clones of MCF7 cells were selected following CRISPR KO of AFDN, SCRIB or both AFDN/SCRIB with two different guide sequences. Western blot with antibodies recognizing endogenous AFDN or SCRIB confirmed the proteins are not expressed. Source data are provided in the Source Data files. b Model of the SCRIB-AFDN-RAS GTPase complex based on our data. c KRAS does not complex with SCRIB in the absence of AFDN. FLAG-SCRIB was expressed in AFDN KO MCF7 cells together with EGFP-KRAS-G12V. Following IP with anti-FLAG, activated KRAS did not co-precipitate with SCRIB unless cells were also expressing EGFP-AFDN. Source data are provided in the Source Data files. d SCRIB is internalized and demonstrates a punctate pattern in AFDN KO MCF7 cells, which do not form cell-cell contacts (top). Expression of EGFP-KRAS-G12V in these cells does not alter SCRIB localization (bottom). Scale bars represent 10 µm. e AFDN remains predominantly at sites of cell contact in SCRIB KO MCF7 cells, which grow in multi-cell layers (top). EGFP-KRAS-G12V expression results in AFDN internalization and breakdown of cell-cell contacts (bottom). Scale bars represent 10 µm.
Fig 2: RAS GTPases co-localize with AFDN.a Expression of wild-type KRAS does not alter AFDN or SCRIB localization. EGFP-KRAS was expressed in MCF7 cells, and localization of endogenous AFDN and SCRIB was detected by immunostaining and confocal microscopy. Projections of z-stacks are at right and top. Position of associated z projections are shown with dashed yellow lines in the merged image. Scale bars represent 10 µm. b KRAS-G12V co-localizes with AFDN and SCRIB in MCF7 cells. Following expression of EGFP-KRAS-G12V, endogenous AFDN and SCRIB were detected by immunostaining. Cells expressing KRAS are found in the monolayer (1), detaching from the monolayer (2) or on top of the monolayer (3). Projections of z-stacks are at right and bottom of the merged images and their position is marked with a dashed yellow line. Scale bars represent 10 µm. c Activated RAS GTPases recruit AFDN to the cell membrane. Cherry-AFDN was co-expressed with EGFP-tagged RAS or RAP GTPases in HeLa cells. EGFP alone or EGFP-tagged wild-type KRAS with AFDN were controls, as was Cherry alone with activated KRAS-G12V. Scale bars represent 10 µm.
Fig 3: Co-localization of SCRIB and AFDN is dependent on their PDZ1 and FHA domains, respectively.a Expression of EGFP-AFDN in AFDN KO MCF7 cells restores endogenous SCRIB localization at cell contacts. SCRIB is also recruited to cell contacts in AFDN KO cells expressing EGFP-AFDN?FHA but appears poorly co-localized with AFDN. Projections of z-stacks are at right and top and their position is marked with a dashed yellow line in the merged image. Scale bars represent 10 µm. b EGFP-SCRIB co-localizes with endogenous AFDN in SCRIB KO MCF7 cells. EGFP-SCRIB?PDZ1 is also enriched at cell contacts in SCRIB KO cells. Projections of z-stacks are at right and top and their position is marked with a dashed yellow line in the merged image. Scale bars represent 10 µm. c z-stack projections along a 10 µm length of cell contacts show exogenous, wild-type AFDN and endogenous SCRIB localization overlaps in AFDN KO cells (top, 3 independent cells). Conversely, expressed AFDN?FHA remains apical to endogenous SCRIB (bottom). d z-stack projections show exogenous, wild-type SCRIB and endogenous AFDN co-localize in SCRIB KO cells (top) while SCRIB?PDZ1 remains basal to endogenous AFDN (bottom).
Fig 4: AFDN FHA and SCRIB PDZ1 interact directly in a phospho-independent manner.a GST-FHA domains with distinct C-termini precipitate full-length SCRIB from lysate. b Lack of positively charged residues on the surface of the AFDN FHA domain. The electrostatic surface of MDC1 FHA domain (left) shows the position of K73 and R58, necessary for binding pThr (PDBid 3UNN). Electrostatic surface of the AFDN FHA domain (PDBid 1WLN) reveals no positive charges (right; dashed circle). Electrostatic potential was calculated using APBS and PyMol. c AFDN and SCRIB co-IP following incubation with phosphatase. EGFP-SCRIB and FLAG-AFDN were co-expressed and precipitated with anti-FLAG following treatment with phosphatase. d Purified SCRIB PDZ1 can interact directly with GST-tagged AFDN FHA. Fragments end at FHA domain residues 501-590 and could only bind SCRIB PDZ1 if extended past residue 580. GST alone was a control. e 1H/15N-HSQC spectra of the AFDN FHA domain. The extended FHA domain (371-580, black) overlays with the core FHA domain (371-514, red) and exhibits several additional peaks. The same peaks are broadened upon addition of unlabeled SCRIB PDZ1 (right, blue), verifying importance of the extended C-terminal region to SCRIB binding. f The extended C-terminal FHA region alone does not bind SCRIB. Mixing PDZ1 domain of SCRIB with GST-tagged FHA domain fragments revealed neither the core FHA domain (371-531) nor C-terminal extension (531-580) in isolation complex with PDZ1. g ITC established AFDN FHA binds SCRIB PDZ1 with a Kd of 14.8 µM, comparable to most PDZ domain interactions. h Plot of peak intensity ratios from 1H/15N-HSQC spectra of PDZ1 alone vs PDZ1 in the presence of 2-fold molar excess AFDN FHA domain. Widespread broadening was observed, most impacted are labelled by residue number (over 6X difference, dashed red line). i Surface representation of the amino acids in SCRIB PDZ1 involved in binding the AFDN FHA domain (PDBid 5VWK). Residues demonstrating the most broadening are coloured red, moderate broadening in orange and no broadening in grey. Unassigned residues are white. Position of the ß-PIX peptide is characteristic of PDZ interactions with C-terminal motifs. All source data are provided in the Source Data files.
Fig 5: Loss of AFDN or SCRIB disrupts ERK and AKT activation kinetics and cell motility in a growth factor-dependent manner.Activation of the MAPK and PI3K pathways following EGF stimulation of MCF7 parental (a), AFDN KO (b) or SCRIB KO (c) cells. pERK or pAKT Western blots measure pathway activity 2.5, 5, 15, 30 or 45 minutes after addition of EGF. Total ERK and AKT Western blots confirmed expression and serve as loading controls. Quantification of MAPK (d; ERK activation) and PI3K (e; AKT activation) activity following addition of EGF over time. Center is the mean and error bars SD as derived from n = 3 independent replicates. f Effect of AFDN or SCRIB KO on cell proliferation compared to parental MCF7 cells over a 72-hour time course from n = 4 independent replicates. Center represents mean and error bars SD. **P < 0.005 (P = 0.003), ns = not significant (P = 0.298) as measured by two-way ANOVA. g KO of SCRIB or AFDN results in motility defects in response to EGF. Wound closure (%) was measured at 24, 48, 72 or 96 hours for MCF7 (P = 0.0001 within group), AFDN KO (P = 0.0145), SCRIB KO (P = 0.0001), and AFDN/SCRIB KO (P = 0.0001) cells. 100 ng/ml EGF was supplemented to the media at time 0 and every 24-hour interval thereafter. n values denote independent replicates. Line represents the median, box the IQR and whiskers min/max. ***P < 0.001, *P < 0.01 as measured by RM one-way ANOVA (within group) or two-way ANOVA (between group). P = < 0.0001 for all between group comparisons. h Phase contrast images of MCF7, SCRIB KO and AFDN KO cells during wound closure. 100 ng/ml EGF was supplemented to the media every 24 hours. Inset are enlarged images of cells at the wound edge. Scale bar represents 100 µm. i AFDN and SCRIB KO cells do not polarize towards the wound. Cells were stained for GM130 (Golgi), which typically orients towards the wound at the leading edge (see parental MCF7 cells; top). Cells were fixed 24 hours after wounding and EGF stimulation. Arrows indicate direction of motility. Scale bars represent 20 µm. All source data are provided in the Source Data files.
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