Fig 1: LCZ696 rescues MI-induced histopathological changes in myocardial cells and improves the inflammatory response in rats. (A) M-mode ultrasonic examination and LV function analysis (n=24). (B) Infarct size, as detected by TTC staining. (C) H&E staining of myocardial tissues (scale bar = 50 µm). (D) Interstitial fibrosis by Masson's staining (scale bar=100 µm). (E) CVF (%) as determined by Masson's staining. (F) Levels of IL-1 ß and IL-18 in the serum of experimental rats, as detected by ELISA. Data are given as mean ± standard deviation, *P<0.05, ns; P>0.05. LVIDs: left ventricular systolic internal dimension. LCZ696, sacubitril/valsartan; MI, myocardial infarction; TTC, triphenyltetrazolium chloride; H&E, hematoxylin and eosin; CVF, collagen volume fraction; LVEF, left ventricular ejection fraction; LVFS, left ventricular fractional shortening, as determined by the following formula: (LVIDd-LVIDs)/LVIDd ×100%; CVF was calculated as follows: CVF=collagen area/view area ×100%.
Fig 2: mRNA levels of inflammatory mediators in lipopolysaccharide (LPS)-induced RAW264.7 cells after organic light-emitting diode (OLED) irradiation. (A) Experimental timeline for examination of the relative mRNA expression levels of interleukin (IL)-6, tumor necrosis factor-a (TNF-a), IL-1 ß, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) revealed by quantitative real-time PCR after OLED irradiation of LPS-stimulated cells. The relative mRNA levels of IL-6 (B), TNF-a (C), IL-1ß (D), iNOS (E) and COX-2 (F) are shown. OLED irradiation significantly reduced the mRNA levels of inflammatory mediators. n = 3; * p < 0.05, *** p < 0.001 (LPS+OLED group vs. LPS-only group).
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