Fig 1: RLCs selectively differentiate to intraglomerular mesangial cells during EC model.A. Representative confocal microscopy images for day 0 and day 7 of β-gal and the EC markers ERG (upper panels) or CD31 (lower panels) co-stained kidney slices; B. Representative confocal microscopy images for day 0 and day 7 of β-gal and the mesangial cell markers PDGFβR (upper panels) or α8-integrin (lower panels) co-stained kidney slices; C. Representative confocal microscopy images for day 0 and day 7 of β-gal/WT1 (podocyte marker) co-stained kidney slices. 4′,6-diamidino-2-phenylindole (DAPI) was used as a nuclear marker throughout. The channels for green (β-gal) and red (corresponding cell marker) fluorescent signals in the dashed squares on day 7 are separately shown in the small right panels. Scale bars correspond to 25 μm.
Fig 2: Mesangial cells and not podocytes display considerable transient damage in the course of the EC model.Mice were analyzed at baseline (day 0), day 1, and day 7 following disease induction. A. Quantification of glomerular mesangial injury by α8-integrin (mesangial cell marker) staining of kidney slices. Data are presented as mean ± SEM, n = 5/5/10 for day 0 /day 1 /day 7, respectively. **p<0.01, ***p<0.001 by one-way ANOVA; B. Representative α8-integrin staining images in the course of the EC model. Scale bars correspond to 25 μm; C. Quantification of podocyte depletion and injury on kidney slices by WT1 and nephrin staining, respectively. Data are presented as mean ± SEM, n = 5/5/10 for day 0 /day 1 /day 7, respectively. *p<0.05, **p<0.01, ns- not significant by one-way ANOVA; D. Representative WT1/nephrin co-staining images in the course of the EC model. 4′,6-diamidino-2-phenylindole (DAPI) was used as a nuclear marker. Scale bars correspond to 25 μm.
Supplier Page from Abcam for Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (Alexa Fluor® 647)