Fig 1: TIPE2 inhibits autophagy levels in H9c2 cells. (A) Levels of autophagy were measured using a tandem sensor. OGD increased autophagy levels in H9c2 cells, while the overexpression of TIPE2 reduced autophagy levels in H9c2 cells with/without OGD. (B) Western blotting was used to analyze the proteins expression levels of autophagy-related LC3B and ATG14 in H9c2 cells. Data are representative of three independent repeats per experiment. &P>0.05 vs. plasmid; *P<0.05, ***P<0.001 vs. plasmid; #P<0.001 vs. plasmid + OGD. TIPE2, TNF-a-induced protein 8-like protein 2; OGD, oxygen glucose deprivation; over-, overexpression; ATG14, autophagy related 14.
Fig 2: Overexpression of TIPE2 reduces OGD-induced H9c2 cell apoptosis. (A) Western blotting was used to analyze the expression levels of TIPE2 in H9c2 cells. (B and C) TUNEL staining was used to detect OGD-induced apoptosis in H9c2 cells. Data are representative of three independent repeats per experiment. ***P<0.001 vs. plasmid. TIPE2, TNF-a-induced protein 8-like protein 2; OGD, oxygen glucose deprivation; over-, overexpression.
Fig 3: TIPE2 overexpression suppressed invasion and induced apoptosis in esophageal carcinoma cells. Transwell assay was employed to assess invasion capability in Ad-V- or Ad-TIPE2-infected EC9706 (a) and EC109 (b) cells. Cell apoptosis rates were measured in Ad-V- or Ad-TIPE2-infected EC9706 (c) and EC109 (d) cells at the 3th day after infection, *P < 0.05
Fig 4: TIPE2 overexpression inhibited tumor growth in vivo. Xenograft tumor models were established by injecting with EC9706 cells infected with Ad-V or Ad-TIPE2 into the flank region of nude mice. a The tumor volume was monitored every 3 days for 24 days. b Resected xenograft tumor tissues were processed to perform IHC assay and H&E staining. c The representative photographs of xenograft tumor tissues were obtained and the mean weight of resected xenograft tumors was measured at the 24th day after infection, *P < 0.05
Fig 5: TIPE2 reduces autophagy and OGD-induced apoptosis in H9c2 cells by activating the mTORC1 signaling pathway. (A) Western blotting was used to analyze the protein expression levels of TIPE2, Raptor, p-mTOR/mTOR and p-4EBP1/4EBP1 in H9c2 cells following different treatments. (B) Semi-quantification of the expression levels presented in part (A). (C) Expression levels of autophagy-related proteins, LC3B and ATG14, in H9c2 cells following different treatments. (D) TUNEL staining was used to detect OGD-induced apoptosis in H9c2 cells. Data are representative of three independent repeats per experiment. *P<0.05, **P<0.01, ***P<0.001 vs. plasmid; #P<0.05 and ###P<0.001 vs. over-TIPE2; &P<0.05 vs over-TIPE2 + ODG. TIPE2, TNF-a-induced protein 8-like protein 2; OGD, oxygen glucose deprivation; over-, overexpression; ATG14, autophagy related 14; p-, phosphorylated; Raptor, regulatory associated protein of mTOR complex 1; 4EBP1, eukaryotic translation initiation factor 4E.
Supplier Page from Abcam for Anti-TIPE2 antibody