Fig 2: The inverse relationship between cell-cell junction level and EMT program in ICC patients(A) Gene expression level of the EMT biomarkers (CDH1, CTNNB1) in HBV-ICC and non-HBV-ICC patients. Data are represented as medians and interquartile ranges.(B) Epithelial cell abundance in HBV-ICC patients and non-HBV-ICC patients. Data are represented as medians and interquartile ranges.(C) The relationship between epithelial cell abundance and CDH1 expression level.(D) The relationship between CCJ score and VIM expression level.(E) The relationship between CCJ score and epithelial cell proportion in our dataset.(F) The relationship between CCJ score and epithelial cell proportion in TCGA datasets.(G) Immunohistochemistry of cell junction and EMT-related proteins TJP1, CTNNB1, and VIM in representative HBV-HCC and non-HBV-ICC patients. Scale bars, 50 µm. More than five fields each under 40× and 100× magnification were examined.