Fig 2: Differential recovery of the phosphorylation level of Lhcb1 and Lhcb2 in multiple mutants. (A) The level of phosphorylation of the LHCII antenna and the core protein PsbA/D1 of PSII was assessed by immunoblotting with antibodies specifically recognizing the phosphorylated peptides. As a control, the total accumulation of the protein was also assessed by immunoblotting. (B) The phosphorylation level of Lhcb1 and Lhcb2 in 15 day-old plantlets was measured by quantification of the immunoblot signal after separation by Phos-tag PAGE. The average level of phosphorylation under 100 µmol photons m-2 s-1 is reported with its standard deviation (n = 3). The phosphorylation levels in the mutants is different from the WT, p values of a Welch T-test of the difference between the double (stn7/STN8/pph1/PBCP) and the triple (stn7/STN8/pph1/pbcp) mutants are shown.

Fig 3: A comparison of the relative abundance of nuclear-encoded chloroplast-targeted photosynthesis transcripts and chloroplast-encoded photosynthesis transcripts in the leaves of CYS, PC, and WT plants grown under moderate light (LL) and high light (HL) conditions. The CYS, PC, and WT plants were grown under moderate light (250 µmol m–2 s–1) for 5 weeks. Thereafter, half of the plants were transferred to HL (800 µmol m–2 s–1) for 8 h, while the remaining half were maintained for 8 h at 250 µmol m–2 s–1. The amounts of transcripts were measured in the leaves of the CYS, PC, and WT plants harvested between 7 h and 8 h into the photoperiod. LHCA, light-harvesting Chl a-binding protein A; LHCB1, light-harvesting Chl a/b-binding protein B1; LHCB2, light-harvesting Chl a/b-binding protein B2; rbcS, the small subunit of Rubisco; psbA, PSII D1 protein; psbD, PSII D2 protein. Bars represent means ±SD (n=24 individual plants per line). Statistical significance is indicated by asterisks: *P-value =0.05, **P-value =0.01.