Fig 1: rTsPmy maintains majority of Tregs in an effective suppressor status (CD62LloCD44hi eTregs) with greater expression of functional phenotypes of TIGIT and CTLA4 in cLP with DSS-induced colitis. Flow cytometry results showed the frequencies of CD44hiCD62Llo cells (A) and TIGIT+ cells (B) in CD4+Foxp3+ T cells of cLP from rTsPmy or PBS-treated mice with or without DSS colitis. The blank control of antibodies of CD62L and CD44 (A) was shown, and the antibody of TIGIT (B) were cut in FMO control samples. The corresponding percentages are shown on the right (n = 5). (C) The frequency of Foxp3+CTLA4+ cells in CD3+CD4+ T cells. The antibody of CTLA4 was cut in FMO control samples. The corresponding percentage is shown on the right (n = 3–5). The bars represent the mean ± SEM. *p < 0.05. cLP, colonic lamina propria.