Fig 1: LPSlow-macrophage injections influence the chemokine network in cGvHD mice. (A, B) Flow cytometric analysis of splenic CD4+ and CD8+ T-cells CXCR3 and CCR5 expression at day 40. (C, D) Flow cytometric analysis of dermal CD4+ and CD8+ T-cells CCR4 and CCR10 expression at day 40. Gating strategy is represented in Supplementary Figure S4. Data represent the mean fluorescence intensity of the chemokine receptors expression on T cells obtained from at least n = 7 biologically independent mice. The ANOVA test with Bonferroni correction was used to detect significant differences between the groups. NS, non-significant; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001. (E, F) RT-qPCR assessment of ccl17 and ccl27 mRNA levels in the skin (E) and cxcl2 and ccl2 mRNA levels in the liver (F) of cGvHD mice at day 40 of the experiment. Results were expressed as fold increase versus the syngeneic group, derived from at least n = 7 biologically independent mice. Unpaired T-test was used to detect the significance between the groups. NS, non-significant; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001.