Alexa Fluor(R) 647 anti-HA.11 Epitope Tag 682404 from BioLegend

Product Specs
ItemAlexa Fluor(R) 647 anti-HA.11 Epitope Tag
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number682404
Quantity100 µg
ApplicationsFlow Cytometry (FC), Immunocytochemistry (ICC)
Concentration0.50 mg/mL
Conjugate/TagAlexa Fluor® 647
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
IsotypeIgG1, Kappa
Clone16B12
HostMouse
Antibody TypePrimary
ImmunogenMonoclonal antibody HA.11 was raised against the twelve amino acid peptide CYPYDVPDYASL
ClonalityMonoclonal
TargetHA.11

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Nuclear translocation of mitochondrial dehydrogenases as an adaptive cardioprotective mechanismNature communicationsJuly 19, 2023Srivastava S, Gajwani P, Jousma J, Miyamoto H, Kwon Y, Jana A, Toth PT, Yan G, Ong SG, Rehman J.Anti-flag tag (1:200) (SIGMA, F7425), Anti-V5 tag (1:200) (Abcam, ab9137), Anti-HA tag (1:400) (BioLegend, 682404) in buffer A (2% BSA in 1x PBS with 0.05% Tween-20) overnight. The cells were washed with wash buffer

(2) Chronic UCN2 treatment desensitizes CRHR2 and improves insulin sensitivityNature CommunicationsJuly 4, 2023Stephen E. Flaherty, Olivier Bezy, Wei Zheng, Dong Yan, Xiangping Li, Srinath Jagarlapudi, Bina Albuquerque, Ryan M. Esquejo, Matthew Peloquin, Meriem Semache, et al.washing 3 times with PBS, cells were kept in the Alexa Fluor 647 anti-HA (1:2000 dilution, BioLegend cat# 682404) diluted in 5% normal goat serum in PBS. Two hours following antibody incubation at room temperature,

(3) Comprehensive functional characterization of SGCB coding variants predicts pathogenicity in limb-girdle muscular dystrophy type R4/2EJournal of Clinical InvestigationJune 15, 2023Chengcheng Li, Jackson Wilborn, Sara Pittman, Jil Daw, Jorge Alonso-Pérez, Jordi Díaz-Manera, Conrad C. Weihl, Gabe Hallerpermeabilized. These cells were then stained for cell surface expression using an anti-HA antibody (Biolegend, 682404/16B12) followed by staining with an Alexa Fluor 647 goat anti-mouse secondary antibody (Thermo Fisher Scientific

(4) Structure and thiazide inhibition mechanism of the human Na–Cl cotransporterNatureFebruary 23, 2023Minrui Fan, Jianxiu Zhang, Chien-Ling Lee, Jinru Zhang, Liang Fengvariants were analyzed by staining cells using anti-hemagglutinin (HA) tag antibody (catalog number 682404; BioLegend). An HA epitope with flanking “SGSGG” sequence on both sides was inserted into extracellular

(5) A comprehensive survey of coronaviral main protease active site diversity in 3D: Identifying and analyzing drug discovery targets in search of broad specificity inhibitors for the next coronavirus pandemicbioRxivFebruary 3, 2023Joseph H. Lubin, Samantha G. Martinusen, Christine Zardecki, Cassandra Olivas, Mickayla Bacorn, MaryAgnes Balogun, Ethan W. Slaton, Amy Wu Wu, Sarah Sakeer, Brian P. Hudson, et al.labeled epitope tag-specific antibodies (anti-FLAG PE, Biolegend, cat# 637309), anti-HA Alexa 647, cat #682404) and incubated at room temperature in the dark for 90 minutes. Stained cells were washed with PBS BSA

(6) G protein-coupled receptor 151 regulates glucose metabolism and hepatic gluconeogenesisNature communicationsDecember 1, 2022Bielczyk-Maczynska E, Zhao M, Zushin PH, Schnurr TM, Kim HJ, Li J, Nallagatla P, Sangwung P, Park CY, Cornn C, et al.Albumin/PBS) overnight, followed by staining with anti-HA antibody conjugated to AlexaFluor647 (BioLegend, #682404, 1:1000) and Hoechst (1:2000) in blocking solution overnight. Finally, cells were washed three times

(7) Biparatopic nanobodies targeting the receptor binding domain efficiently neutralize SARS-CoV-2iScienceNovember 18, 2022Phillip Pymm, Samuel J. Redmond, Olan Dolezal, Francesca Mordant, Ester Lopez, James P. Cooney, Kathryn C. Davidson, Ebene R. Haycroft, Chee Wah Tan, Rebecca Seneviratna, et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Anti-HA-AF647 clone 16B12,Biolegend,Cat#682404; RRID: AB_2566616 Anti-human IgG-PE,Southern Biotech,Cat#9040–09; RRID: 2796601 AffiniPure sheep anti-mouse

(8) Sequences in the cytoplasmic tail of SARS-CoV-2 Spike facilitate expression at the cell surface and syncytia formationNature CommunicationsDecember 1, 2021Jérôme Cattin-Ortolá, Lawrence G. Welch, Sarah L. Maslen, Guido Papa, Leo C. James, Sean MunroBuffer and incubated in Cyto-Fast Wash Buffer containing an anti-HA AF647 conjugate (1:1000, BioLegend, 682404) and an anti-FLAG AF549 conjugate (for cells expressing FLAG-tagged M; 1:1000, Biolegend, 637314) for

(9) HIV-1 Nef interacts with the cyclin K/CDK13 complex to antagonize SERINC5 for optimal viral infectivityCell reportsAugust 10, 2021Chai Q, Li S, Collins MK, Li R, Ahmad I, Johnson SF, Frabutt DA, Yang Z, Shen X, Sun L, et al.AB_2536530 Goat-anti-mouse-IgG-HRP,Thermo Fisher,Cat#G21040; RRID: AB_2536527 AF647 anti-HA,BioLegend,Cat#682404; RRID: AB_2566616 Pacific Blue anti-HA,BioLegend,Cat#901525; RRID: AB_2734659 Monoclonal anti-Nef,"Zheng

(10) Tracking the motion of the K _V 1.2 voltage sensor reveals the molecular perturbations caused by a de novo mutation in a case of epilepsyThe Journal of PhysiologyNovember 1, 2020Antonios Pantazis, Maki Kaneko, Marina Angelini, Federica Steccanella, Annie M. Westerlund, Sarah H. Lindström, Michelle Nilsson, Lucie Delemotte, Sulagna C. Saitta, Riccardo Olceseanti-HA-conjugated Alexa Fluor 647 (Alexa Fluor 647 anti-HA.11 Epitope Tag Antibody; BioLegend Cat. No. 682404, RRID:AB_2566616): first, cells were incubated in 100 μl Zombie Violet (1:500, PBS) for 15 min at room

(11) Olfactory Stimulation Regulates the Birth of Neurons That Express Specific Odorant ReceptorsCell ReportsOctober 6, 2020Carl J. van der Linden, Pooja Gupta, Ashraful Islam Bhuiya, Kelci R. Riddick, Kawsar Hossain, Stephen W. Santoropolyclonal),Abcam,Cat# ab9110; RRID: AB_307019 Alexa647-anti-HA antibodies (mouse monoclonal),Biolegend,Cat# 682404; RRID: AB_2566616 "Chemicals, Peptides, and Recombinant Proteins",, "DIG RNA Labeling Mix, 10 × conc.",Roche,Cat#

(12) Butyrophilin-like proteins display combinatorial diversity in selecting and maintaining signature intraepithelial γδ T cell compartmentsNature communicationsJuly 28, 2020Jandke A, Melandri D, Monin L, Ushakov DS, Laing AG, Vantourout P, East P, Nitta T, Narita T, Takayanagi H, et al.PerCPCy5.5,SK7,BioLegend,344808,1:300 DYDDDDK PeCy7,L5,BioLegend,637324,1:300 HA AF647,16B12,BioLegend,682404,1:200 HIS PE,J095G46,BioLegend,362603,1:100 CD24-BV650,M1/69,BD,563545,1:400 CD62L-BV421,MEL-14,BioLegend,104436,1:300 CD44-Pe-Cy7,IM-7,BioLegend,103030,1:300 CD45

(13) Butyrophilin-like 3 Directly Binds a Human Vγ4⁺ T Cell Receptor Using a Modality Distinct from Clonally-Restricted AntigenImmunityNovember 19, 2019Willcox CR, Vantourout P, Salim M, Zlatareva I, Melandri D, Zanardo L, George R, Kjaer S, Jeeves M, Mohammed F, et al.(clone 16B12),Biolegend,Cat#901502; LOT B242905; RRID: AB_2565007 HA-AF647 (clone 16B12),Biolegend,Cat#682404; LOT B246404; RRID: AB_2566616 Mouse IgG1 isotype control (clone MOPC-1),Biolegend,Cat#400166; LOT

(14) Natural HIV-1 Nef Polymorphisms Impair SERINC5 Downregulation ActivityCell reportsNovember 5, 2019Jin SW, Alsahafi N, Kuang XT, Swann SA, Toyoda M, Göttlinger H, Walker BD, Ueno T, Finzi A, Brumme ZL, et al.,BD Biosciences,Cat # 555349; RRID: AB_398593 HA.11 Epitope Tag antibody (Alexa Fluor® 647),BioLegend,Cat # 682404; RRID:AB_2566616 "HIV-1 Core Antigen, Mouse clone KC57 (RD1)",Beckman Coulter,Cat # 6604667 "Human

(15) PD-L1 checkpoint blockade delivered by retroviral replicating vector confers anti-tumor efficacy in murine tumor modelsOncotargetMarch 19, 2019Mitchell LA, Yagiz K, Hofacre A, Viaud S, Munday AW, Espinoza FL, Mendoza D, Rodriguez-Aguirre ME, Bergqvist S, Haghighi A, et al.2 sets. One set of cells was stained with Alexa Fluor 647-conjugated anti-HA antibody (BioLegend, # 682404) and the second set of cells was stained with PE-conjugated anti-human PD-L1 antibody (ebioscience,

(16) Interferon-Induced Transmembrane Protein 1 Restricts Replication of Viruses That Enter Cells via the Plasma MembraneJournal of virologyMarch 15, 2019Smith SE, Busse DC, Binter S, Weston S, Diaz Soria C, Laksono BM, Clare S, Van Nieuwkoop S, Van den Hoogen BG, Clement M, et al.quantiﬁed by ﬂow cytometry using an anti-HA antibody conjugated to Alexa Fluor 647 (catalog number 682404; BioLegend). Cells were washed in PBS and harvested by trypsinization. Cells were washed in 3% FBS–PBS and

(17) SIVcol Nef counteracts SERINC5 by promoting its proteasomal degradation but does not efficiently enhance HIV-1 replication in human CD4+ T cells and lymphoid tissuePLoS pathogensAugust 1, 2018Kmiec D, Akbil B, Ananth S, Hotter D, Sparrer KMJ, Stürzel CM, Trautz B, Ayouba A, Peeters M, Yao Z, et al.and Permeabilization Kit and stained with anti-HA antibody conjugated to Alexa Fluor 647 (BioLegend #682404). To measure surface SERINC5 levels, unpermeabilized cells were stained in the same manner before fixation

(18) Structural Basis of Smoothened Activation in Hedgehog SignalingCellJuly 12, 2018Huang P, Zheng S, Wierbowski BM, Kim Y, Nedelcu D, Aravena L, Liu J, Kruse AC, Salic A.2010",N/A Mouse anti-acetylated tubulin,Sigma,Cat# T7451 "Mouse anti-HA, Alexa647-labeled",BioLegend,Cat# 682404 "Chemicals, Peptides, and Recombinant Proteins","Chemicals, Peptides, and Recombinant Proteins","Chemicals,

(19) Comparison of γ-Aminobutyric Acid, Type A (GABAA), Receptor αβγ and αβδ Expression Using Flow Cytometry and Electrophysiology: EVIDENCE FOR ALTERNATIVE SUBUNIT STOICHIOMETRIES AND ARRANGEMENTSThe Journal of biological chemistrySeptember 23, 2016Botzolakis EJ, Gurba KN, Lagrange AH, Feng HJ, Stanic AK, Hu N, Macdonald RL.

(20) The pH of prostatic fluid: a reappraisal and therapeutic implicationsTransactions of the American Association of Genito-Urinary SurgeonsJanuary 1, 1977W R Fair, J J Cordonnier

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Alexa Fluor(R) 647 anti-HA.11 Epitope Tag from BioLegend

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Alexa Fluor(R) 647 anti-HA.11 Epitope Tag from BioLegend

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