Fig 1: Evaluation of IgG subclasses, IgA and IgM elicited in immunized mice. 10-fold dilutions of pre-immunized (pre-bleed, Black circle) and immunized mice sera collected after last immunization (week 10), were evaluated in ELISA to assess the IgG subtypes; (A) IgG1, (B) IgG2a, (C) IgG2b, (D) IgG2c, (E) IgG3, as well as (F) IgA, and (G) IgM induced with no adjuvant (Black square), with Alum (Blue square), or with GLA-SE (Red square). (H) The half-max binding for each individual mouse sera sample, identified by an individual number, for the terminal bleed was calculated and displayed for IgG1, IgG2a, and IgG2b. n = 6 mice for each group. **** represents statistical significance of P < 0.0001, ***P < 0.001, **P < 0.01, and *P < 0.05. Blue stars represent the difference between ID2 alone and ID2 + Alum, red stars represent differences between ID2 alone and ID2 + GLA-SE and purple stars represent differences between ID2 + Alum and ID2 + GLA-SE.