PD-1 (EH33) Mouse mAb 43248S from Cell Signaling Technology

Product Specs
ItemPD-1 (EH33) Mouse mAb
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number43248S
Quantity100 µl
ApplicationsIHC-Bond, IHC-P
ReactivityH
TargetPDCD1
HostMouse
NCBI Full Gene Nameprogrammed cell death 1
NCBI Gene AliasesCD279, PD-1, PD1, SLEB2, hPD-1, hPD-l, hSLE1
ClonalityMonoclonal
Research AreaImmunology and Inflammation

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Dysregulated Repeat Element Viral-like Immune Response in Hepatocellular CarcinomabioRxivDecember 5, 2023Avril K. Coley, Chenyue Lu, Amaya Pankaj, Matthew J. Emmett, Evan R. Lang, Yuhui Song, Katherine H. Xu, Nova Xu, Bidish K. Patel, Abhijit Chougule, et al.ER1 95C TIM3 (D5D5R),Cell Signaling,Cat #45208,1:400,20min ER2 95C PD1 (EH33),Cell Signaling,Cat #43248,1:200,20min ER2 95C PDL1 (E1L3N),Cell Signaling,Cat #13684,1:200-1:400,20min ER2/ER1 95C

(2) Creatine riboside is a cancer cellâ€“derived metabolite associated with arginine auxotrophyJournal of Clinical InvestigationJuly 15, 2022Amelia L. Parker, Leila Toulabi, Takahiro Oike, Yasuyuki Kanke, Daxeshkumar Patel, Takeshi Tada, Sheryse Taylor, Jessica A. Beck, Elise Bowman, Michelle L. Reyzer, et al.Opal Polaris 620); and programmed cell death 1 (PD-1) (primary Ab: Cell Signaling Technology, catalog 43248; secondary Ab: Opal Polaris 570) as outlined in Supplemental Methods. Slides were imaged on a Vectra

(3) High Expression of TIM 3 and Galectin 9 on Immunohistochemistry Staining of Tumor Specimen at Diagnosis in Pediatric Patients with Ewing SarcomaJ Cancer Immunol (Wilmington)June 30, 2022Stephanie J. Si, Gerald B. Wertheim, David M. BarrettRetrieval,Protocol CD20,L26,Dako,M0755,1:1K,E2-20,Leica Bond Refine CD68,Kp1,Dako,M0814,1:4K,E2-20,Leica Bond Refine PD1,EH33,Cell Signaling,43248,1:100,E2-20,Leica Bond Refine Tim-3,D5DR,Cell Signaling,45208,1:50,E2-20,Leica Bond Refine Lag-3,17B4,LSBio,C18692,1:200,E1-20,Leica

(4) ERBB2D16 Expression in HER2 Positive Gastric Cancer Is Associated With Resistance to TrastuzumabFrontiers in OncologyApril 7, 2022Shuo Wang, Yuze Zhao, Yuguang Song, Guoliang Qiao, Yan Di, Jing Zhao, Pingping Sun, Huixia Zheng, He Huang, Hongyan Huangantibodies applied in this study were as follows: anti-CD3 (Abnova MAB9626, 1:100, Opal 690), PD-1 (CST, #43248, 1:200, Opal 620), PD-L1 (CST, #13684, 1:200, Opal 650), E-cadherin (CST, #3195, 1:500, Opal 650), and

(5) Unique characteristics of tertiary lymphoid structures in kidney clear cell carcinoma: prognostic outcome and comparison with bladder cancerJournal for ImmunoTherapy of CancerMarch 21, 2022Tsukasa Masuda et al.antibody (#M0784, Dako), anti-FOXP3 antibody (to label Treg cells, #ab20034, Abcam), anti-PD-1 antibody (#43248, Cell Signaling Technology), and anti-PD-L1 antibody (#13684, Cell Signaling Technology). Immunohistochemical staining

(6) Pharmacological disruption of the MTDH–SND1 complex enhances tumor antigen presentation and synergizes with anti-PD-1 therapy in metastatic breast cancerNature CancerJanuary 1, 2022Minhong Shen et al.98941S, 1:100 dilution for mouse samples), anti-MTDH (Sigma, AMAB90762, 1:500 dilution), anti-PD-1 (CST, 43248S, 1:200 dilution), anti-CD3 (abcam, Ab16669, 1:200 dilution), anti-CD4 (CST, 25229S, 1:200 dilution),

(7) Myeloid antigen-presenting cell niches sustain antitumor T cells and license PD-1 blockade via CD28 costimulationCancer CellDecember 1, 2021Jaikumar Duraiswamy, Riccardo Turrini, Aspram Minasyan, David Barras, Isaac Crespo, Alizée J. Grimm, Julia Casado, Raphael Genolet, Fabrizio Benedetti, Alexandre Wicky, et al.,Cell Signalling,"Cat# 11882, RRID:AB_2687824" "Mouse monoclonal anti-PD-1, Clone EH33 (tCycIF)",Cell Signalling,"Cat# 43248, RRID:AB_2728836" "Rabbit monoclonal anti-PD-L1, Clone E1L3N (tCycIF)",Cell Signalling,"Cat# 13684,

(8) Bevacizumab improves tumor infiltration of mature dendritic cells and effector T-cells in triple-negative breast cancer patientsnpj Precision OncologyDecember 1, 2021Yves Boucher et al.CD11c (Leica CD11C-563-L-CE, 1:1500), CD8 (Agilent Dako M710301, 1:5000), PD-1 (Cell Signaling Technology 43248 S, 1:11000), granzyme-B (Dako M7235, 1:100), CD4 (Dako M731029, 1:250), FOXP3 (BioLegend 320102, 1:2000), CD31

(9) Immune Characters and Plasticity of the Sentinel Lymph Node in Colorectal Cancer PatientsJournal of Immunology ResearchAugust 18, 2021Xiaoyun Li, Jingling Tang, Hang Du, Xinjun Wang, Liyun Wu, Pingsheng Hu, Hua Zhang, Ruyi Zhang, Yuan YangCell Signaling Technology, 70306), Foxp3 (1 : 500, Abcam, 20034), PD1 (1 : 50, Cell Signaling Technology, 43248), and CD163 (1 : 100, Cell Signaling Technology, 93498). The slides were incubated with the primary

(10) miRNA-34a-5p regulates progression of neuroblastoma via modulating the Wnt/β-catenin signaling pathway by targeting SOX4Medicine (Baltimore)May 21, 2021Yue Wang, Enqing Guan, Dehua Li, Lirong Sunprimary antibodies at 4 °C overnight. The following primary antibodies were used: N-Cadherin (CST, #43248, 1:200, Opal 620), E-Cadherin (CST, #3169, 1:500, Opal 650), Anti-Vimentin (Abcam, ab92574, 1:400, Opal

(11) BDNF expression in GISTs predicts poor prognosis when associated with PD-L1 positive tumor-infiltrating lymphocytesOncoimmunologyJanuary 1, 2021Tian GA, Xu WT, Sun Y, Wang J, Ke Q, Yuan MJ, Wang JJ, Zhuang C, Gong Q.antibodies used were as follows: BDNF (GeneTex, GTX132621, 1: 200), PD-L1 (CST, # 13684, 1: 200), PD-1 (CST, 43248, 1: 100), TIM3 (Abcam, ab185703, 1: 200), Galectin 9 (Abcam, ab69630, 1: 200), CD3 (Servicebio, GB13440,

(12) The prognostic significance of immune microenvironment in breast ductal carcinoma in situBritish Journal of CancerMay 12, 2020Michael S. Toss, Asima Abidi, Dorothea Lesche, Chitra Joseph, Sakshi Mahale, Hugo Saunders, Tanjina Kader, Islam M. Miligy, Andrew R. Green, Kylie L. Gorringe, et al.FOXP3 (mouse monoclonal [236A/E7), Abcam (ab20034)), PD1 (Mouse monoclonal [EH33] Cell signalling (43248S)), PDL1 (Rabbit monoclonal antibody [E1L3N] Cell signalling (13684S)) were used to characterise TILs

(13) The Association Between Immune Characteristic and Clinical Pathology in Chinese Patients with Adenocarcinoma of Esophagogastric JunctionCancer Management and ResearchMay 11, 2020Yangyang Wang, Shuchang Wang, Chunchao Zhu, Hui Cao, Zizhen Zhang, Enhao Zhaoon the TMA using antibodies speciﬁc to PD-L1 (1: 100, Abcam, UK, ab205921), PD-1 (1: 100, CST,USA, 43248), Tim-3 (1:200, CST,USA, 45208), Gal-9 (1:250, Abcam, UK, ab69630), CD3 (1: 200, Wuhan Goodbio Technology

(14) The immune checkpoint molecules PD-1, PD-L1, TIM-3 and LAG-3 in diffuse large B-cell lymphomaOncotargetMarch 12, 2019Benjamin J. Chen, Ravi Dashnamoorthy, Pallavi Galera, Vladislav Makarenko, Hong Chang, Srimoyee Ghosh, Andrew M. Evens(clone E1L3N, #13684, Cell Signaling Technologies (CST), Danvers, MA, USA), anti-PD-1 (clone EH33, #43248, CST), anti-TIM-3 (clone D5D5R, #45208, CST), and anti-LAG-3 (clone 17B4, #40466, Abcam, Cambridge,

(15) Profiling the Tumour Immune Microenvironment in Pancreatic Neuroendocrine Neoplasms with Multispectral Imaging Indicates Distinct Subpopulation Characteristics Concordant with WHO 2017 ClassificationScientific reportsSeptember 3, 2018Takahashi D, Kojima M, Suzuki T, Sugimoto M, Kobayashi S, Takahashi S, Konishi M, Gotohda N, Ikeda M, Nakatsura T, et al.Fukuoka, Japan; KT022, 1:200 dilution), anti-human PD-1 (EH33, Cell Signaling Technology, Danvers, USA; #43248S, 1:200 dilution), anti-human PD-L1 (clone E1L3N, Cell Signaling Technology; #13684P, 1:1200 dilution),

(16) Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopeseLifeJuly 11, 2018Lin JR, Izar B, Wang S, Yapp C, Mei S, Shah PM, Santagata S, Sorger PK.,R and D,AF6228,Polyclonal,N/D,AB_10971818 anti-MITF,MITF,†,Abcam,AB12039,C5,N/D,AB_298801 anti-Ncad,N-Cadherin,†,Abcam,AB18203,Polyclonal,N/D,AB_444317 anti-NCAM,NCAM,†,Abcam,AB6123,ERIC-1,N/D,AB_2149537 anti-NF1,NF1,†,Abcam,AB178323,McNFn27b,N/D, anti-pCTD,Pol II CTD(S2),†,Active Motif,61083,3E10,N/D,AB_2687450 anti-PD1,PD1,†,CST,43248,EH33,N/D, anti-pTuberin,pTuberin(S664),†,Abcam,AB133465,EPR8202,N/D,AB_11157389 anti-S100,S100,†,Dako,Z0311,Polyclonal,N/D,AB_10013383 anti-SIRT3,SIRT3,†,CST,2627,C73E3,N/D,AB_2188622 anti-TIA1,TIA1,†,Santa Cruz

(17) PD-L1 Expression and CD8⁺ T Cell Infiltration Predict a Favorable Prognosis in Advanced Gastric CancerJournal of Immunology ResearchMay 29, 2018Yangyang Wang, Chunchao Zhu, Wei Song, Jun Li, Gang Zhao, Hui Caothe TMA using antibodies speciﬁc to PD-L1 (1 : 100, Abcam, UK, ab205921), PD-1 (1 : 100, CST, USA, 43248), CD3 (1 : 200, Wuhan Goodbio Technology Co., Ltd., China), CD8 (1 : 100, Wuhan Goodbio Technology Co.,

(18) PD-L1 Expression Predicts a Distinct Prognosis in Krukenberg Tumor with Corresponding OriginsJournal of Immunology ResearchMay 8, 2018Haiyan Tai, Qin Yang, Zhiyong Wu, Su’an Sun, Rui Cao, Yanfeng Xi, Ran Zhao, Mengyu Zhang, Zhigang Zhang, Congjian Xu(rabbit monoclonal antibody, CST, USA, 78701, 1 : 200), anti-PD-1 (mouse monoclonal antibody, CST, USA, 43248, 1 : 100), antiFOXP3 (rabbit monoclonal antibody, CST, USA, 98377, 1 : 50), anti-CD3 (rabbit monoclonal

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J Cancer Immunol (Wilmington)

PD-1 (EH33) Mouse mAb from Cell Signaling Technology

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Cell Signaling Technology

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PD-1 (EH33) Mouse mAb from Cell Signaling Technology

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