Anti-SOX17 antibody [OTI3B10] ab84990 from Abcam

Product Specs
ItemAnti-SOX17 antibody [OTI3B10]
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab84990
Antibody TypePrimary
CloneOTI3B10
Conjugate/TagUnconjugated
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Western blot
IsotypeIgG1
ClonalityMonoclonal
Quantity100 UL
HostMouse, Nude mouse
FormatPurified from mouse ascites fluids by affinity chromatography
Concentration1 mg/ml
Research AreaEpigenetics
Gene NameSox17
ReactivityHuman
TargetSOX17

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Generation, characterization, and differentiation of induced pluripotent stem-like cells in the domestic catJournal of Reproduction and DevelopmentDecember 8, 2023Ryoji KANEGI, Shingo HATOYA, Kazuto KIMURA, Kyohei YODOE, Toshiya NISHIMURA, Kikuya SUGIURA, Noritoshi KAWATE, Toshio INABAstaining. Primary antibodies against tubulin-β-III-isoform (1:1000; MAB1637; Millipore), SOX17 (1-µg/ml; ab84990; Abcam), and desmin (1-µg/ml; ab82506; Abcam) were used. The secondary antibodies used were goat anti-mouse

(2) Single-cell analyses reveal distinct expression patterns and roles of long non-coding RNAs during hESC differentiation into pancreatic progenitorsStem Cell Research & TherapyMarch 13, 2023Hai-Tao Luo, Qian He, Wei Yang, Fei He, Jun Dong, Chao-Feng Hu, Xiao-Fei Yang, Ning Li, Fu-Rong Liincubated with 10% BSA and 0.5% Triton X-100 in PBS for 1 h. Primary antibodies (anti-SOX17, Abcam/ab84990, 1:1000, and anti-FOXA2, R&D/AF2400, 1:500) were then added and incubated at 4 °C overnight. The next

(3) A reference human induced pluripotent stem cell line for large-scale collaborative studiesCell stem cellDecember 1, 2022Pantazis CB, Yang A, Lara E, McDonough JA, Blauwendraat C, Peng L, Oguro H, Kanaujiya J, Zou J, Sebesta D, et al.anti-Nestin,Abcam,ab18102; RRID:AB_444246 Goat anti-brachyury,R&D Systems,AF2085; RRID:AB_2200235 Mouse anti-SOX17,Abcam,ab84990; RRID:AB_1861437 Goat anti-FOXA2,R&D Systems,AF2400; RRID:AB_2294104 Rabbit anti-Lmx1,Merck Millipore,AB10533;

(4) LRP1 is a neuronal receptor for α-synuclein uptake and spreadMolecular neurodegenerationSeptember 2, 2022Chen K, Martens YA, Meneses A, Ryu DH, Lu W, Raulin AC, Li F, Zhao J, Chen Y, Jin Y, et al.Nanog (Cell Signaling, cat# 4903, 1: 300), TRA-1–60 (Abcam, cat# ab16288, 1: 300), Sox17 (Abcam, cat# ab84990, 1: 300), Brachyury (R&D, cat# AF2085, 1: 300), Nestin (Abcam, cat# ab18102, 1:300), and Tuj1 (Sigma-Aldrich,

(5) Autophagy modulates cell fate decisions during lineage commitmentAutophagyAugust 1, 2022Sharma K, Asp NT, Harrison S, Siller R, Baumgarten SF, Gupta S, Chollet ME, Andersen E, Sullivan GJ, Simonsen A.antibody,Sigma-Aldrich,"T8660, RRID:AB_477590" FOXA2,Abcam,"ab40874, RRID:AB_732411" SOX17 antibody [3B10],Abcam,"ab84990, RRID:AB_1861437" MAP1LC3B,Cell Signaling Technology,"2775, RRID:AB_915950" Mouse Anti-Human LAMP1

(6) Micro-RNA-124-5p promotes insulin producing cell differentiation through regulating transcriptional factor NKX6.1Biochemistry and Biophysics ReportsJuly 1, 2022Xianjun Zhang, Shunzi Shao, Xijiang Zhao, Meng Zhang, Jingbo WangX-100 in PBS for 1 h. Subsequently, cells were incubated with primary antibodies (anti-SOX17 Abcam ab84990 1:1000, anti-FOXA2 R&D AF2400 1:500, anti-NKX6.1 Abcam ab221549 1:1000, anti-insulin Abcam ab181547

(7) Phenotypical, functional and transcriptomic comparison of two modified methods of hepatocyte differentiation from human induced pluripotent stem cellsBiomedical reportsMay 1, 2022Li R, Zhao Y, Yourick JJ, Sprando RL, Gao X.monoclonal antibody (cat. no. ab108422; Abcam; 1:50), SOX17 mouse anti-human monoclonal antibody (cat. no. ab84990; Abcam; 1:50), hepatocyte nuclear factor 4 α (HNF4A) rabbit anti-human polyclonal antibody (cat. no.

(8) The combination of dextran sulphate and polyvinyl alcohol prevents excess aggregation and promotes proliferation of pluripotent stem cells in suspension cultureCell proliferationSeptember 1, 2021Tang X, Wu H, Xie J, Wang N, Chen Q, Zhong Z, Qiu Y, Wang J, Li X, Situ P, et al.refreshed every 2 days. After two weeks, the EBs were stained for SOX17 (anti‐SOX17 mouse IgG, 1:800, Cat # ab84990, Abcam), Brachyury (anti‐Brachyury Rabbit IgG, 1:800, Cat # 81694S, CST) and GFAP (anti‐GFAP Chicken

(9) Generation and validation of APOE knockout human iPSC-derived cerebral organoidsSTAR protocolsJune 18, 2021Martens YA, Xu S, Tait R, Li G, Zhao XC, Lu W, Liu CC, Kanekiyo T, Bu G, Zhao J.2C1.3A11),Abcam,Cat#: ab18102; RRID: AB_444246 Mouse monoclonal anti-Sox17 (clone 3B10),Abcam,Cat#: ab84990; RRID: AB_1861437 Goat polyclonal anti-Brachyury,R&D Systems,Cat#: AF2085; RRID: AB_2200235 Rabbit

(10) Enhanced structure and function of human pluripotent stem cell-derived beta-cells cultured on extracellular matrixStem cells translational medicineMarch 1, 2021Singh R, Cottle L, Loudovaris T, Xiao D, Yang P, Thomas HE, Kebede MA, Thorn P.Following primary antibodies were used for the staining purposes: Sox2 (Abcam; ab97959), Sox17 (Abcam; ab84990), Nkx6.1 (R&D Systems; AF5857), Pdx1 (R&D Systems; AF2419), Insulin (DAKO, A0564), Glucagon (Sigma,

(11) IFI16 promotes human embryonic stem cell trilineage specification through interaction with p53npj Regenerative MedicineDecember 1, 2020Qian He, Zubiao Wu, Wei Yang, Doukou Jiang, Chaofeng Hu, Xiaofei Yang, Ning Li, Furong Liwas purchased from Abcam (ab5790, Western blots/Immunofluorescence), SOX17 was purchased from Abcam (ab84990, Western blots/ Immunofluorescence), SNAI2 was purchased from Santa Cruz (sc-166476, Immunofluorescence),

(12) APOE4 exacerbates synapse loss and neurodegeneration in Alzheimer's disease patient iPSC-derived cerebral organoidsNature communicationsNovember 2, 2020Zhao J, Fu Y, Yamazaki Y, Ren Y, Davis MD, Liu CC, Lu W, Wang X, Chen K, Cherukuri Y, et al.(Cell Signaling, 4903, 1:300), TRA-1-60 (Abcam, ab16288, 1:300), SSEA4 (Abcam, ab16287, 1:300), Sox17 (Abcam, ab84990, 1:300), Brachyury (R&D, AF2085, 1:300), Nestin (Abcam, ab18102, 1:500), Sox2 (Abcam, ab97959, 1:500),

(13) Efficient Generation and Correction of Mutations in Human iPS Cells Utilizing mRNAs of CRISPR Base Editors and Prime EditorsGenes (Basel)May 6, 2020Duran Sürün, Aksana Schneider, Jovan Mircetic, Katrin Neumann, Felix Lansing, Maciej Paszkowski-Rogacz, Vanessa Hänchen, Min Ae Lee-Kirsch, Frank Buchholzfixation, permeabilization, blocking, and staining with MS mAb to SOX17 (3B10) primary antibody (Abcam, ab84990, Cambridge, UK) and Alexa Fluor 488 goat anti-mouse IgG. Nuclei were counterstained with NucBlue™ Fixed Cell ReadyProbes™ Reagent

(14) Multipotent vascular stem cells contribute to neurovascular regeneration of peripheral nerveStem Cell Research & TherapyDecember 1, 2019Ching-Wen Huang, Yuan-Yu Hsueh, Wen-Chin Huang, Shyam Patel, Song Licell markers, samples were incubated with specific primary antibodies: Sox10 (ab155279, Abcam), Sox17 (ab84990, Abcam), Ki67 (ab197234, Abcam), smooth muscle α-actin (⍺-SMA) (ab5694, Abcam), calponin-1 (CNN1) (ab233854,

(15) TCam-2 Cells Deficient for SOX2 and FOXA2 Are Blocked in Differentiation and Maintain a Seminoma-Like Cell Fate In VivoCancers (Basel)May 25, 2019Daniel Nettersheim, Saskia Vadder, Sina Jostes, Alena Heimsoeth, Hubert Schorle,Santa Cruz,H-10,Mouse,-,1:400 PRDM1,H.M. Jäck,-,Rabbit,1:200,- SOX2,R&D systems,MAB2018,Mouse,1:200,1:200 SOX17,Abcam,ab84990,Mouse,1:400,- SOX17,R&D systems,AF1924,Goat,-,1:1000 TFAP2C,Santa Cruz,6E4/4,Mouse,1:200,- Secondary

(16) Distribution of label-retaining cells and their properties in the vocal fold mucosaLaryngoscope investigative otolaryngologyFebruary 1, 2019Sato K, Kurita T, Chitose SI, Sato K, Umeno H, Yano H.desmin (Abcam, Cambridge, UK, ab6322, mouse monoclonal), a 1:100 against Sox17 (Abcam, Cambridge, UK, ab84990, mouse monoclonal), a 1:250 against CD44 (Abcam, Cambridge, UK, ab157107, rabbit polyclonal), a 1:200

(17) SOX17 Inhibits Tumor Metastasis Via Wnt Signaling In Endometrial CancerOncoTargets and therapyJanuary 1, 2019Zhou W, Wang K, Wang J, Qu J, Du G, Zhang Y.to the manufacturer’s instructions with the following antibodies speciﬁc for target proteins: SOX17 (ab84990; Abcam, Cambridge, UK), E-cadherin (ab15148; Abcam), and β-catenin (ab16051; Abcam). Scoring was performed

(18) Differentiation of hepatocyte-like cells from human pluripotent stem cells using small moleculesDifferentiation; research in biological diversityMay 1, 2018Asumda FZ, Hatzistergos KE, Dykxhoorn DM, Jakubski S, Edwards J, Thomas E, Schiff ER.Kit (395B-1KT) was from Sigma (St. Louis, MO). Antibodies against OCT4 (ab19857), SOX2 (ab92494), SOX17 (ab84990) were from Abcam (Cambridge, MA). Antibodies against NANOG (sc-293121), SSEA-1 (sc-21702), TRA-160 (sc-21705),

(19) Genetic Correction and Hepatic Differentiation of Hemophilia B-specific Human Induced Pluripotent Stem CellsChinese Medical Sciences JournalSeptember 27, 2017何琼 et al.antibodies Antigen Distributor Host Cat# Dilution OCT3/4 Santa Cruz Rabbit sc-9081 1:500 SOX17 ABCAM Mouse Ab84990 1:50-100 AFP ABCAM Rabbit ab169552 1:100-250 ALB R&D System Mouse MAB1455 8-25 μg/ml F IX

(20) Effects of Aminoglycoside Antibiotics on Human Embryonic Stem Cell Viability during Differentiation In VitroStem cells internationalJanuary 1, 2017Varghese DS, Parween S, Ardah MT, Emerald BS, Ansari SA.

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Anti-SOX17 antibody [OTI3B10] from Abcam

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Anti-SOX17 antibody [OTI3B10] from Abcam

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