Fig 1: Oxidation of peroxiredoxins (Prx) in response to exogenous or DAO-derived H2O2 or menadione. (A): Representative redox western blot (30 µg protein) for Prx1, Prx2, Prx3, and Prx-SO3 after stimulation with different concentration of D- or L-Ala in HUVEC. (B): Quantification of redox-western blots by densitometry (n = 3). (C): Redox western blot and quantification (D) for Prx1 and Prx2 with HUVEC-DAO pre-incubated with auranofin (20 min, 3 µM) prior D- or L-ala stimulation, * p < 0.05 as compared to CTL with auranofin. One-way-ANOVA with Bonferroni correction.