WT1 Antibody AF5729 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemWT1 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberAF5729
NCBI Gene AliasesWIT-2, GUD, WT33, AWT1, WT-1, NPHS4, WAGR
ReactivityHuman
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
ApplicationsWestern Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready
HostGoat
IsotypeIgG
TargetWT1
NCBI Full Gene NameWT1 transcription factor
ClonalityPolyclonal
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ImmunogenE. coli-derived recombinant human WT1, Met127-Gly249, Accession # P19544
ConcentrationLYOPH
PurityAntigen Affinity-purified
Brand/TrademarkR&D Systems, a Bio-Techne Brand
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Specificity: Detects human WT1 in direct ELISAs and Western blots

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Improved epicardial cardiac fibroblast generation from iPSCsJournal of molecular and cellular cardiologyMarch 1, 2022Whitehead AJ, Hocker JD, Ren B, Engler AJ.fibronectin EDA (NBP1–51723, Novus Bio, 1:200) to confirm matrix competency. WT1 (R&D Systems, Cat. AF5729, Minneapolis, MN) and vimentin (Cell Signaling Technology, Cat. 5741, Danvers, MA) were used as epicardial

(2) Unravelling the Role of PAX2 Mutation in Human Focal Segmental GlomerulosclerosisBiomedicinesDecember 1, 2021Longaretti L, Trionfini P, Brizi V, Xinaris C, Mele C, Breno M, Romano E, Giampietro R, Remuzzi G, Benigni A, et al.System,MAB861,1:50 Paxillin,Genetex,GTX125891,1:200 Synaptopodin,Abcam,ab101883,1:100 TLE4,Santa Cruz,SC-365406,1:150 WT1,R&D System,AF5729,1:50 ZO-1,Invitrogen,61-7300,1:100 List of primary antibodies.

(3) Differentiation of human iPSCs into functional podocytesPloS oneJanuary 1, 2018Rauch C, Feifel E, Kern G, Murphy C, Meier F, Parson W, Beilmann M, Jennings P, Gstraunthaler G, Wilmes A.(podocin, Sigma P0372) at 1:500, anti-NPHS1 (nephrin, Abcam ab183099) at 1:75 and WT-1 (R&D Bio-techne, AF5729) at 1:100, and incubated at RT for 30 min. The secondary antibody Alexa Fluor 568 (Thermofisher; Goat:

(4) Renal progenitors derived from human iPSCs engraft and restore function in a mouse model of acute kidney injuryScientific reportsMarch 6, 2015Imberti B, Tomasoni S, Ciampi O, Pezzotta A, Derosas M, Xinaris C, Rizzo P, Papadimou E, Novelli R, Benigni A, et al.

(5) A novel source of cultured podocytesPloS oneJanuary 1, 2013Da Sacco S, Lemley KV, Sedrakyan S, Zanusso I, Petrosyan A, Peti-Peterdi J, Burford J, De Filippo RE, Perin L.

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WT1 Antibody

WT1 Antibody

WT1 Antibody

WT1 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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WT1 Antibody from R&D Systems, a Bio-Techne Brand

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