Fig 1: Probing the mechanism of purinosome formation in hypoxia. a, The effect of hypoxia (24 h) on the transcription of HSP70-2, HSP70-1, and HSP90 measured by qPCR. Data shown are n = 3, mean ± S.E. b, the effect of hypoxia (24 h) on the protein expression of HSP90, HSP70-1, and HSP70-2. c, the association of endogenous ADSL and HSP70-2 in normoxic and hypoxic cells (both in purine-rich medium) or normoxic cells in purine-depleted medium (24 h). The red spots (PLA signal, exemplar shown by white arrow) arise from interacting proteins, whereas the DAPI-stained nuclei are in blue. Scale bar = 25 μm, uncropped images are deposited in the raw data files. d, quantifying the number of cells with purinosomes marked with FGAMS-mCherry in hypoxia (24 h) with (+ siRNA) or without siRNA to HSP70–2 (− siRNA). Data shown are n = 3, mean ± S.E., total number of cells counted are shown in parentheses. e, the effect of HSP70–2 overexpression on purinosome formation in cells using FGAMS-mCherry in normoxia (24 h). Data shown are n = 3, mean ± S.E., total number of cells counted are shown in parentheses. f, the effect of the glycolysis inhibitor 2-DG and the PPP inhibitor 6-AN on purinosome formation in normoxia and hypoxia (6 h). Both molecules revert the number of purinosome-containing cells in hypoxia to normoxic levels, with no effect on normoxic cells. Data shown are n = 3, mean ± S.E., total number of cells counted are shown in parentheses.