Fig 1: Immunofluorescent staining for myelin, neurons and phagocytic cells in spinal cord sections from 21-23-week-old P361R-SMA mice.Staining for CNPase, a myelin-associated protein (a), neuron-specific ßIII-Tubulin (ßIII-Tub) (b) along with a marker of lysosomal lumen, CathepsinD (CatD) in spinal cord sections derived from wild-type (WT) and P361R-SMA mice. c Astrocytes (GFAP) and lysosomes (CatD) were immunostained in spinal cord sections from WT and P361R-SMA mice. d Serial sections from spinal cords of WT and P361R-SMA mice were stained with luxol fast blue (LFB), or immunohistochemically stained for phagocytic macrophages/microglia (Mac-2) and CatD. The insets highlight a macrophage with LFB staining. Scale bars represent 500 µm (left) and 100 µm (right) for each of WT and P361R-SMA. Higher magnifications are shown focusing on the dorsal funiculus. Scale bar for the insets represents 10 µm.