Western blot analysis of RRAD using RRAD Polyclonal Antibody (Product # PA5-37885) (0.5 µg/mL) in staining of Lane A: HEK293 lysates overexpressing several HA-tagged Mouse GTPases, including Rrad (10 µg protein in RIPA buffer) and compared with an HA-specific antibody. Lane B: (0.5 µg/mL) staining of WT and KO lysates of Mouse Heart (100 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data provided by Prof. D Andres, University of Kentucky College of Medicine, USA.
Western blot of RAD1/RRAD was performed using A) HEK293 lysates overexpressing several HA-tagged mouse GTPases (including Rrad) and B) 100 µg of WT and KO lysates of mouse heart. Lysates were probed with an HA-specific antibody (A) and a RAD1/RRAD polyclonal antibody (Product # PA5-37885) (B) at a dilution of 0.5 µg/mL and incubated for 1 hour, followed by chemiluminescent detection.
Supplier Page from Thermo Fisher Scientific for RRAD Antibody