Fig 1: Comparison of measured and predicted protein abundances relative to wildtype for manA variants in S. Typhimurium. Measured ManA levels are weighted averages of mass spectrometry measurements (3 biological replicates, 3 digestion replicates, and 3 technical replicates each) and Western blots (3 to 5 replicates each), which correlate well (cf. Supplementary Fig. S24). Predicted protein levels are given in terms of the protein expression score relative to wildtype. The protein expression score is based on the function estimates shown in Fig. 3 setting the weight v6 for transcript levels equal to zero and the weights for all other features equal to one, because transcripts levels are undetermined at the time of sequence proposal.