Fig 2: Expression levels of chloroplast-encoded genes in osgun4 and osgun5 mutants under NF or Lin treatment.(A and B) qRT–PCR assay showing the relative expression of several representative PEP-transcribed genes (psaA, psaB, psbA, and rbcL) in the osgun4(A) and osgun5(B) mutants under NF or Lin treatment. Values are means ± SD of three replicates. Two asterisks (**) mark statistically significant differences from the wild type with the same treatment (t test, p = 0.01). UBIQUITIN was used as an internal control.(C and D) qRT–PCR assay showing the relative expression of several representative NEP-transcribed genes (rpoA, rpoB, rpoC1, and rpoC2) in the osgun4(D) and osgun5(E) mutants under NF or Lin treatment.Values are means ± SD of three replicates. Two asterisks (**) mark statistically significant differences from the wild type with the same treatment (t test, p = 0.01). UBIQUITIN was used as an internal control.

Fig 3: Expression levels of chloroplast-encoded genes in the Arabidopsis gun4 and gun5 mutants under NF or Lin treatment.(A and B) qRT–PCR assay showing the relative expression of several representative PEP-transcribed genes (psbA, psbD, psbN, psaJ, and rbcL) and NEP-transcribed genes (clpP, rpoB, and rpoC1) in the Arabidopsis gun4(A) and gun5(B) mutants under NF or Lin treatment.Values are means ± SD of three replicates. Two asterisks (**) mark statistically significant differences from the wild type with the same treatment (t test, p = 0.01). ACTIN was used as an internal control.