PEPC antibody - Phosphoenolpyruvate carboxylase AS09 458 from Agrisera

Product Specs
ItemPEPC antibody - Phosphoenolpyruvate carboxylase
CompanyAgrisera
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberAS09 458
ImmunogenKLH-conjugated synthetic peptide well conserved PEPC1 and sequences from different plant species including Arabidopsis thaliana Q9MAH0, At1g53310 (PEPC 1), Q84VW9, At3g14940 (PEPC 3). The peptide chosen to elicit this antibody is also perfectly conserved in bacterial type of this enzyme NP_177043.2 (PEPC 4). For Zea mays, the peptide is converved in PEP1 and PEP4 isoforms
TargetPEPC
ReactivityAnanas comosus, Arabidopsis thaliana, Cenchrus ciliaris, Chloris gayana, Chromera velia, Cyanthobasis fruticulosa, Hordeum vulgare, Jatropha curcas, Kochia prostrata, Leptochloa fusca, Lupinus, Megathyrsus maximus, Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Panicum antidotale, Panicum coloratum, Petrosimonia nigdeensis, Pinus strobus, Saccharum spp. hybrid clone C91-301, Salsola lanata, Salsola laricifolia,Salsola grandis, Salsola tragus Sorghum bicolor, Synechocystis PCC 6803, Phaeodactylum tricornutum, Pinus strobus, Thalassiosira weissfloggi, Zea mays, Zostera muelleri
ClonalityPolyclonal
HostRabbit
ApplicationsImmunolocalization, Western blot
FormatLyophilized
NCBI Gene AliasesATPEPC1, F12M16_21, PEP(PHOSPHOENOLPYRUVATE) CARBOXYLASE 1, phosphoenolpyruvate carboxylase 3, phosphoenolpyruvate carboxylase 1, F12M16.21, ATPPC1, PEPC1, ATPPC3
Research AreaPlant Biology
PurityImmunogen affinity purified serum in PBS pH 7.4
StorageStore lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Please do not re-use this primary antibody solution. In case of cyanobacterial samples there will be no signal in your second incubation
Molecular Weight110 : 105 kDa

Specifications/Features

1 : 1000 with standard ECL (WB), 1: 500 (IL)

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Agrisera

Box 57

Vännäs

Sweden

Phone: +46 (0) 935 33033

Email: info@agrisera.com
orders@agrisera.com

Company Profile

Website: http://www.agrisera.com

(1) S-acylated and nucleus-localized SALT OVERLY SENSITIVE3/CALCINEURIN B-LIKE4 stabilizes GIGANTEA to regulate Arabidopsis flowering time under salt stressThe Plant CellJanuary 2, 2023Hee Jin Park, Francisco M Gámez-Arjona, Marika Lindahl, Rashid Aman, Irene Villalta, Joon-Yung Cha, Raul Carranco, Chae Jin Lim, Elena García, Ray A Bressan, et al.antibodies against α-Histone3 (Abcam, #ab1791) and α-Phospho Enol Pyruvate Carboxylase (PEPC; Agrisera, #AS09 458) were used as nuclear and cytosolic markers. For the acyl resin-assisted capture (acyl-RAC) of SOS3,

(2) Intensity and duration of salinity required to form adaptive response in C4 halophyte Kochia prostrata (L.) ShradFrontiers in Plant ScienceOctober 7, 2022Elena Shuyskaya, Zulfira Rakhmankulova, Maria Prokofieva, Luizat Saidova, Kristina Toderich, Pavel VoroninRubisco at a dilution of 1:10,000 (RbcL, AS03 037, Agrisera, Sweden), PEPC at a dilution of 1:5,000 (AS09 458, Agrisera, Sweden) for 1 h. Immunoreactions were detected using peroxidase-conjugated anti-rabbit IgG

(3) Identification of Pri-miRNA Stem-Loop Interacting Proteins in Plants Using a Modified Version of the Csy4 CRISPR EndonucleaseInternational Journal of Molecular SciencesAugust 11, 2022Janina Lüders, Andreas R. Winkel, Marlene Reichel, Valentin W. Bitterer, Marion Scheibe, Christiane Widmann, Falk Butter, Tino Köster(Agrisera, Vännäs, Sweden, AS10710; rabbit; dilution 1:5000), PHOSPHOENOLPYRUVATE CARBOXYLASE (Agrisera AS09458; rabbit; dilution 1:20,000) and SERRATE (Agrisera AS09532A; rabbit; dilution 1:1000). Secondary antibody

(4) CPK28-NLP7 module integrates cold-induced Ca ²⁺ signal and transcriptional reprogramming in ArabidopsisScience AdvancesJuly 1, 2022Yanglin Ding, Hao Yang, Shifeng Wu, Diyi Fu, Minze Li, Zhizhong Gong, Shuhua Yangagainst GFP (TransGen, HT801-01), cytoplasmic marker phosphoenolpyruvate carboxylase (PEPC) (Agrisera, #AS09 458; RRID: AB_1312), and nuclear marker histone H3 (Millipore, #05-499; RRID: AB_2787688). Microscale thermophoresis MST

(5) Production of succinate by engineered strains of Synechocystis PCC 6803 overexpressing phosphoenolpyruvate carboxylase and a glyoxylate shuntMicrobial Cell FactoriesDecember 1, 2021Claudia Durall, Kateryna Kukil, Jeffrey A. Hawkes, Alessia Albergati, Peter Lindblad, Pia LindbergBlocking Agent from Amersham was used and the primary antibody rabbit anti-PEPc (polyclonal from Agrisera (AS09458), diluted 1:300) was incubated overnight at 4 ℃. Supplementary Information

(6) Widespread occurrence of microRNA-mediated target cleavage on membrane-bound polysomesGenome BiologyDecember 1, 2021Xiaoyu Yang, Chenjiang You, Xufeng Wang, Lei Gao, Beixin Mo, Lin Liu, Xuemei Chenrabbit anti-RPL13 primary antibody (AS13 2650, Agrisera, USA) and rabbit anti-PEPC primary antibody (AS09 458, Agrisera, USA), respectively. The membranes were then incubated with goat anti-rabbit HRP-conjugated

(7) Expression of a CO2-permeable aquaporin enhances mesophyll conductance in the C4 species Setaria viridiseLifeNovember 29, 2021Maria Ermakova, Hannah Osborn, Michael Groszmann, Soumi Bala, Andrew Bowerman, Samantha McGaughey, Caitlin Byrt, Hugo Alonso-cantabrana, Steve Tyerman, Robert T Furbank, et al.(Martin-Avila et al., 2020) (1:10,000), Rieske (AS08 330, 1:3000, Agrisera, Vännäs, Sweden), PEPC (AS09 458, 1:10,000, Agrisera), CA (Azad et al., 2016; Ludwig et al., 1998) (1:10,000). Quantification of immunoblots

(8) Brassinosteroids inhibit miRNA-mediated translational repression by decreasing AGO1 on the endoplasmic reticulumJournal of integrative plant biologyAugust 1, 2021Wang T, Zheng Y, Tang Q, Zhong S, Su W, Zheng B.

(9) DEAD-box helicases modulate dicing body formation in ArabidopsisScience advancesApril 1, 2021Li Q, Liu N, Liu Q, Zheng X, Lu L, Gao W, Liu Y, Liu Y, Zhang S, Wang Q, et al.described previously (76). α-H3 (EASYBIO, BE7004) and α-PEPC phosphoenolpyruvate carboxylase (Agrisera, AS09458) were used as nuclear and cytoplasmic controls, respectively. PlantCV image analysis Color images

(10) Protein arginine methyltransferase 3 fine-tunes the assembly/disassembly of pre-ribosomes to repress nucleolar stress by interacting with RPS2B in arabidopsisMolecular PlantFebruary 1, 2021Runlai Hang, Zhen Wang, Chao Yang, Lilan Luo, Beixin Mo, Xuemei Chen, Jing Sun, Chunyan Liu, Xiaofeng CaoSupplemental Figure 15. The commercial antibodies used include anti-histone H3 (Abcam, ab1791), anti-PEPC (Agrisera, AS09458), and anti-HSC70 (Enzo, ADI-SPA-818-D). Subcellular Localization of RPS2B and Nucleolus/Nucleus Area Measurement For

(11) Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803Biotechnology for BiofuelsDecember 1, 2020Claudia Durall, Pia Lindberg, Jianping Yu, Peter Lindblad

(12) N ⁶ ‐methyladenosine and RNA secondary structure affect transcript stability and protein abundance during systemic salt stress in ArabidopsisPlant DirectJuly 24, 2020Marianne C. Kramer, Kevin A. Janssen, Kyle Palos, Andrew D. L. Nelson, Lee E. Vandivier, Benjamin A. Garcia, Eric Lyons, Mark A. Beilstein, Brian D. Gregoryand salt‐treated INTACT purified nuclei or whole leaf lysate was performed using anti‐PEPC (1:1,000; AS09 458; Agrisera), anti‐CNX1/2 (1:500; AS12 2365; Agrisera) and anti‐H3 (1:1,000; ab1791; Abcam) antibodies.

(13) Genetic variation in ZmTIP1 contributes to root hair elongation and drought tolerance in maizePlant Biotechnology JournalMay 1, 2020Xiaomin Zhang, Yue Mi, Hude Mao, Shengxue Liu, Limei Chen, Feng Qin1 h at 4 °C, 100 000 g to obtain a soluble fraction (S) and a pellet (P). Anti‐PEPC (Agrisera, Cat#AS09458) and anti‐AHA2 antibodies (Yang et al., 2010) were used as cytosolic and membrane fraction markers,

(14) Overexpression of the Rieske FeS protein of the Cytochrome b 6 f complex increases C4 photosynthesis in Setaria viridisCommunications BiologyDecember 1, 2019Maria Ermakova, Patricia E. Lopez-Calcagno, Christine A. Raines, Robert T. Furbank, Susanne von CaemmererLhca1 (Agrisera, AS01 005), Lhcb2 (Agrisera, AS01 003), PsbS (Agrisera, AS09 533), PEPC (Agrisera, AS09 458), RbcL (Agrisera, AS03 037), Cyt f (Agrisera, AS08 306). Quantification of immunoblots was performed

(15) PUB25 and PUB26 Promote Plant Freezing Tolerance by Degrading the Cold Signaling Negative Regulator MYB15Developmental cellOctober 21, 2019Wang X, Ding Y, Li Z, Shi Y, Wang J, Hua J, Gong Z, Zhou JM, Yang S.

(16) TALE‐carrying bacterial pathogens trap host nuclear import receptors for facilitation of infection of riceMolecular Plant PathologyApril 1, 2019Shugang Hui, Yarui Shi, Jingjing Tian, Li Wang, Yueyue Li, Shiping Wang, Meng Yuananti‐FLAG (F7425, Sigma), anti‐Histone H3 (06‐755, Millipore, Burlington, Massachusetts) and anti‐PEPC (AS09458, Agrisera, Vannas, Sweden). Sequence analysis Multiple sequence alignments of amino acid sequences

(17) EGR 2 phosphatase regulates OST 1 kinase activity and freezing tolerance inArabidopsisThe EMBO JournalJanuary 1, 2019Yanglin Ding, Jian Lv, Yiting Shi, Junping Gao, Jian Hua, Chunpeng Song, Zhizhong Gong, Shuhua YangAnti-Myc (Sigma-Aldrich, #M4439) was used to detect EGR2 and EGR2G2A proteins. Anti-PEPC (Agrisera, #AS09 458; RRID: AB_1312) and anti-GHR1 (produced from Shanghai Youke Biotech Co., LTD) antibodies were used to

(18) Overexpression of Rubisco subunits with RAF1 increases Rubisco content in maizeNature PlantsOctober 1, 2018Coralie E. Salesse-Smith, Robert E. Sharwood, Florian A. Busch, Johannes Kromdijk, Viktoriya Bardal, David B. Stern

(19) Shade compromises the photosynthetic efficiency of NADP-ME less than that of PEP-CK and NAD-ME C4 grassesJournal of Experimental BotanyMay 25, 2018Balasaheb V Sonawane, Robert E Sharwood, Spencer Whitney, Oula Ghannoumfor 1 h with membranes at room temperature with gentle agitation. Antiserum raised against PEPC (Cat. AS09 458) was obtained from AgriSera and diluted 1:2000 with TBS. For NADP-ME and PEP-CK, synthetic peptides

(20) Maize leaf PPDK regulatory protein isoform-2 is specific to bundle sheath chloroplasts and paradoxically lacks a Pi-dependent PPDK activation activityJournal of Experimental BotanyFebruary 23, 2018Chris J Chastain, Lisa M Baird, Mitchell T Walker, Charles C Bergman, Gulnara T Novbatova, Candida S Mamani-Quispe, Jim N BurnellAS13 2640), as were affinity-purified polyclonal peptide antibodies against PEP carboxylase (PEPC; cat.# AS09 458) and Rubisco large subunit (LSU; cat.# AS03 037A). The latter two antibodies were used for testing cell

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Anti-PEPC : Phosphoenolpyruvate carboxylase

PEPC antibody - Phosphoenolpyruvate carboxylase from Agrisera

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Specifications/Features

Agrisera

Citations (32)

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PEPC antibody - Phosphoenolpyruvate carboxylase from Agrisera

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