Fig 1: Expression of immunomodulatory proteins and response to checkpoint inhibition in opT cells. (A, B) Expression of checkpoint proteins in opT cells compared with the matched PBMCs by flow cytometry and CyTOF. (C, D) Immuno-staining for MHC-I, MHC-II, PD-L1, HLA-E and CEACAM1 in tumor organoids from Pt3 and Pt38. (E) Changes in interferon-? secretion by opT cells after 48 hours of pretreatment with anti-PD-1, PD-L1 and TIM3 locking antibodies (10 µg/mL) or NKG2A, TIM3, TIGIT and LAG3 protein (2 µg/mL), in the presence or absence of autologous tumor organoids from Pt3. **, p<0.01 compared with opT plus tumor without antibody treatment. P value calculated using two-tailed, unpaired t-test. CyTOF, cytometry by time-of-flight; opT, organoid-primed T; PBMCs, peripheral blood mononuclear cells; Pt, patient.