PE/Dazzle(TM) 594 anti-human CD335 (NKp46) 331930 from BioLegend

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Oncoimmunology
January 01 2021

Fig 1: Gemcitabine does not negatively impact HPC-NK cell phenotype and functionality. A-K. Phenotype of HPC-NK cells treated with gemcitabine for 48 h. Percentage positivity corrected for background staining in the corresponding control sample is shown. Control samples did not contain the antibodies of interest. Protein expression of (a) NKG2A on viable CD56+CD3- cells, or (b) CD158b, (c) CD158e1, (d) NKp30, (e) NKp44, (f) NKp46, (g) DNAM-1, (h) NKG2D, (i) CD69 (all n = 3) on viable CD56+CD3-NKG2A+ cells, or (j) FasL, (k) TRAIL (both n = 3) on living CD56+ cells is shown. L-N. Functionality of HPC-NK cells treated with gemcitabine for 48 h, after 4 h stimulation with (white) or without (black) K562 (all n = 3). Percentage positivity as protein expression of (l) perforin on viable CD56+ cells, (m) CD107a (degranulation) and (n) IFN? on viable CD56+ perforin+ HPC-NK cells is shown. Graphs show individual donors (NK1-6). Statistical significance was tested by repeated-measures One-way ANOVA for A-K and repeated-measures Two-way ANOVA for L-N, all ANOVAs with Bonferroni correction
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Frontiers in immunology
January 01 2022

Fig 2: NK cells maintain the expression of activating receptors after cryopreservation. PM21-NK cells expanded from T-cell-depleted PBMCs were obtained from multiple donors (N = 3–7). Cells were cryopreserved while donor-matched fresh PM21-NK cells (black squares) were maintained in culture. Frozen PM21-NK cells were thawed and rested 16 h the day before analysis (blue triangles) or thawed and rested for 1 h on the day of analysis (gray circles). Expression of NK cell activating receptors CD16, NKG2D, DNAM-1, NKp30, NKp46, NKp44, as well as CD69, granzyme B, and perforin was determined by flow cytometry. No statistically significant difference was seen in the expression of activating receptors either 1 h immediately post-thaw or after a 16-h rest in donor and expansion-matched PM21-NK cells (N = 5–7 donors). Data are presented as a scatter plot with each point representing the average of two technical replicates with donor-pair lines. No statistical significance was determined by multiple paired t-tests with a threshold for significance at p < 0.05.

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PE/Dazzle(TM) 594 anti-human CD335 (NKp46)

The PE/Dazzle(TM) 594 anti-human CD335 (NKp46) Antibody from BioLegend is a Mouse Monoclonal antibody to NCR1. This antibody recognizes Homo Sapiens, and Human antigen. The PE/Dazzle(TM) 594 anti-human CD335 (NKp46) Antibody has been validated for the following applications: Flow Cytometry.

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PE/Dazzle(TM) 594 anti-human CD335 (NKp46) [9E2]

Oncoimmunology

Frontiers in immunology

PE/Dazzle(TM) 594 anti-human CD335 (NKp46) from BioLegend

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BioLegend

Citations (4)

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PE/Dazzle(TM) 594 anti-human CD335 (NKp46) from BioLegend

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