TSC22/TSC22D1 Antibody (OTI4H8) NBP2-46238 from Novus Biologicals, a Bio-Techne Brand

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The Journal of experimental medicine
October 03 2022

Fig 1: Transcription factors regulated lung fibroblast invasion. (A–D) Knockdown of transcriptional factors was confirmed by qRT-PCR (A and B) and Western blotting (C and D). A, n = 9 for FOXF1, n = 4 for CREBRF, TSC22D1, and KLF9, n = 6 for MXI1, n = 3 for NFE2L2; B, n = 4 for HMGA2, n = 5 for DPF3. (E) FOXF1 and SEMA7A expressions showed negative correlation in scRNA-seq by SeqGeq. (F and G) Relative mRNA levels of FOXF1, SEMA7A, and collagen-related protein gene, ACTA2 and COL1A1 (F, n = 5 per group) and cell-surface expression of SEMA7A (G) after FOXF1 knockdown. (H–J) Representative images (H) and index quantification (I and J) of migration and invasion of fibroblasts after knockdown assay. I, FOXF1, CREBRF, TSC22D1, and MXI1, n = 6 for migration and invasion, KLF9, n = 3 for migration and invasion, NFE2L2, n = 3 for migration and n = 11 for invasion; J, HMGA2, n = 6 for migration and invasion, DPF3, n = 3 for migration and invasion. CTL, control; non, noninvasive; in, invasive. Three or four independent experiments were performed on fibroblasts from different patients (A, B, F, I, and J). Data are the mean ± SEM. Scale bar: 1 mm. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 by Student’s t test. Source data are available for this figure: SourceData F4.
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The Journal of experimental medicine
October 03 2022

Fig 2: Differentially expressed transcription factors in invasive and noninvasive fibroblasts. (A) Visualization of differentially expressing transcription factors using violin plot in invasive and noninvasive fibroblasts. (B–D) Expression validation of transcription factors by bulk RNA-seq analysis (B) and qRT-PCR analysis (C and D). B, n = 9 per group; C, n = 4 for TSC22D1 and n = 5 for other groups; D, n = 6 per group. (E and F) Western blot analysis of transcription factor expression in invasive and noninvasive fibroblasts. GAPDH served as loading control. non, noninvasive fibroblasts; in, invasive fibroblasts. Three independent experiments were performed on fibroblasts from different patients (B–D). Data are the mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 by Student’s t test (B–D). Source data are available for this figure: SourceData F3.