Fig 1: Inhibition of SARS-CoV-2 by different types of IFN. (A) Endogenous expression of ZAP and its cofactors KHNYN and TRIM25 in Calu-3 cells that were left untreated and/or uninfected or were treated with the amounts of IFNs indicated in panel B and infected with SARS-CoV-2. Whole-cell lysates were immunoblotted and stained with anti-ZAP, anti-KHNYN, anti-TRIM25, anti-ISG15 and anti-ß-actin. (B) Relative expression levels of the long (L) or short (S) isoforms of ZAP and TRIM25 normalized to unstimulated cells set as 100%. The data were derived from the Western blots shown in panel A. (C) Raw RT-qPCR threshold cycle (CT) values (left) and corresponding SARS-CoV-2 RNA copy numbers per ml (right) in the supernatants of Calu-3 cells were determined as described for panel A. n = 1 in technical duplicate. Shown are mean values ±SD.