Fig 1: Loss of E1 and E3 reduces E protein and GATA3 binding at Ea. (A) Diagram of relative positions of Ta1-Ta4 protein binding regions and GATA3, RUNX1, and E1-E3 E protein binding sites within Ea. ChIP-qPCR of E2A (B), HEB (C), GATA3 (D), and RUNX1 (E) binding in Ea+/+ (n = 3) and Ea?E1?E3/?E1?E3 (n = 3) whole thymocytes. The RAG ASE and Gapdh served as positive and negative controls, respectively. Data were pooled from 3 independent experiments, one of which included an age-matched C57BL/6 as Ea+/+. Data are plotted as mean ± SEM. Statistical analysis: Student’s t test. *p < 0.05, **p < 0.01.