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Goat Anti-Human IgG Antibody, HRP conjugate
Goat Anti-Human IgG Antibody, HRP conjugate from MilliporeSigma
Be the first to write a review!
Citations:
(22)
Supplier Page
Supplier Page from
MilliporeSigma for
Goat Anti-Human IgG Antibody, HRP conjugate
MilliporeSigma's
Goat Anti-Human IgG Antibody, HRP conjugate
is a Goat Polyclonal antibody. This antibody has been shown to work in applications such as: Other.
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Goat Anti-Human IgG Antibody, HRP conjugate
Company
MilliporeSigma
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
AP112P
Immunogen
Prepared from purified human IgG
Brand/Trademark
Chemicon
Source/Expression System
Antibodies
Reactivity
human
Target
Human IgG
Applications
Virus detection: Antibodies for serology assay development
Clonality
Polyclonal
Conjugate/Tag
peroxidase conjugate
Host
goat
Format
Purified by immunoaffinity chromatography using antigen coupled to agarose beads. Lyophilized. Buffer is 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA. Contains no preservative
Clone
polyclonal
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MilliporeSigma
400 Summit Drive
Burlington
,
Massachusetts
01803
United States
Phone
: (800)-645-5476
(800)-645-5439
Company Profile
Website:
www.SigmaAldrich.com/
Citations (22)
(1)
Development of High-Loading Trastuzumab PLGA Nanoparticles: A Powerful Tool Against HER2 Positive Breast Cancer Cells
International Journal of Nanomedicine
November 24, 2023
Tania Mariastella Caputo, Giovannina Barisciano, Chiara Mulè, Angela Maria Cusano, Anna Aliberti, Livio Muccillo, Vittorio Colantuoni, Lina Sabatino, Andrea Cusano
calculating the amounts of proteins loaded in each lane or incubated with an anti-TZ
human
antibody (
AP112P
,
Sigma-Aldrich
Co. Merck KGaA, St. Louis, MO, USA), then washed and images acquired by chemiluminescence
(2)
Distinguishing features of long COVID identified through immune profiling
Nature
November 2, 2023
Jon Klein, Jamie Wood, Jillian R. Jaycox, Rahul M. Dhodapkar, Peiwen Lu, Jeff R. Gehlhausen, Alexandra Tabachnikova, Kerrie Greene, Laura Tabacof, Amyn A. Malik,
et al.
temperature. The plates were washed six times with wash buffer. Goat anti-human IgG Fc HRP (
Sigma-Aldrich
,
AP112P
) diluted
1:10,000
in 1% Omniblock non-fat dry milk in PBS was added to the plates and incubated 1 h
(3)
Autoimmunity to synovial extracellular matrix proteins in patients with postinfectious Lyme arthritis
Journal of Clinical Investigation
September 1, 2023
Korawit Kanjana, Klemen Strle, Robert B. Lochhead, Annalisa Pianta, Laura M. Mateyka, Qi Wang, Sheila L. Arvikar, David E. Kling, Cameron A. Deangelo, Lucy Curham,
et al.
duplicate wells and incubated for 1.5 hours, followed by HRP-conjugated
goat anti-human IgG
(
1:2,000
;
AP112P
,
Sigma-Aldrich
) and then tetramethylbenzidine substrate (BD). All incubations and washes were performed
(4)
Inhibiting the Proliferation of Colorectal Cancer Cells by Reducing TSPO/VDAC Expression
Iranian Journal of Public Health
July 23, 2023
Yang Liu, Shuyue Wang, Weining Yang
VDAC: S-7647R, Bioss), and then incubated with goat anti-rabbit IGG-HRP (
1:5000
,
Millipore
, MA, USA,
AP112P
) for another 2h.
Protein expression
was finally detected by
ECL-Western Blotting Substrate Kit
(
Abnova
,
(5)
MiRNA-206 Affects the Recovery of Sciatic Function by Stimulating BDNF Activity through the Down-regulation of Notch3 Expression
J Musculoskelet Neuronal Interact
March 1, 2023
Meng Wang, Shuang Wu, Jun Wang, Dandan Fan, Zhiyong Li, Shaohua Tian, Sining Yao, Hongyu Zhang, Hongwei Gao
incubated with secondary antibodies including Goat anti-rabbit IgG-HRP (
1:5000
,
Millipore
, MA, USA,
AP112P
) and Goat anti-mouse IgG-HRP (
1:5000
,
Millipore
, MA, USA,
AP126P
) at 37°C for 1 h. Finally,
protein expression
(6)
Quality of antibody responses by adults and young children to 13-valent pneumococcal conjugate vaccination and Streptococcus pneumoniae colonisation
Vaccine
November 28, 2022
Asia-Sophia Wolf, Elena Mitsi, Scott Jones, Simon P. Jochems, Lucy Roalfe, Deus Thindwa, James E. Meiring, Jacquline Msefula, Farouck Bonomali, Tikhala Makhaza Jere,
et al.
alkaline phosphatase (
Southern Biotech
, cat. no.
2040–04
) or
horseradish
peroxidase (
Sigma
, cat. no.
AP112P
). Plates were developed with p-nitrophenyl phosphate (
Sigma
) or 3,3′,5,5′-Tetramethylbenzidine (
TMB) solution
(7)
Therapeutic effect of multiple functional minicircle vector encoding anti-CD25/IL-10/CXCR3 in allograft rejection model
The Korean journal of internal medicine
September 1, 2022
Lim SW, Shin YJ, Cui S, Ko EJ, Yoo SH, Chung BH, Yang CW.
conditioned media or plasma overnight at 4°C. After
incubation
with anti-human immunoglobulin G (IgG)-HRP (
AP112P
,
Merck Millipore
, Billerica, MA, USA) for 2 hours, the TMB substrate solution (
DY999
, R&D Systems) was
(8)
Neutralizing antibody activity against 21 SARS-CoV-2 variants in older adults vaccinated with BNT162b2
Nature Microbiology
August 1, 2022
Joseph Newman et al.
anti-human IgG
horseradish
peroxidase antibody conjugate diluted
1:15,000
(
Sigma-Aldrich
, catalogue no.
AP112P
) and 3,3′,5,5′-tetramethylbenzidine (Europa Bioproducts). We analysed samples in duplicate and evaluated
(9)
Histopathological and Immunological Findings in the Common Marmoset Following Exposure to Aerosolized SARS-CoV-2
Viruses
July 21, 2022
Ireland RE, Davies CD, Keyser E, Findlay JSF, Eastaugh L, Laws TR, Salguero FJ, Hunter L, Nelson M.
Human SARS-CoV-2 Spike (Trimer) Ig Total ELISA Kit
(
BMS2323
,
Thermo Fisher Scientific
, Paisley, UK) with the human IgG conjugate (
AP112P
,
Sigma-Aldrich
, Gillingham, UK), as per manufacturer’s protocol. Briefly, 10 µL of diluted plasma (
1:10
)
(10)
Development of an efficient reproducible cell-cell transmission assay for rapid quantification of SARS-CoV-2 spike interaction with hACE2
Cell Reports Methods
July 18, 2022
George Ssenyange, Maya Kerfoot, Min Zhao, Shelli Farhadian, Sidi Chen, Lei Peng, Ping Ren, Charles S. Dela Cruz, Shaili Gupta, Richard E. Sutton
HRP,
Sigma-Aldrich
,Cat#
12-348
Rabbit anti-GAPDH,Sigma-Aldrich,Cat# SAB5600208 Anti-human HRP,
Sigma-Aldrich
,Cat#
AP112P
Anti-HIV
human
sera,NIH
AIDS
Reagent Repository,Cat# 3957 ,, Biological samples,Biological samples,Biological
(11)
P-selectin glycoprotein ligand-1 (PSGL-1/CD162) is incorporated into clinical HIV-1 isolates and can mediate virus capture and subsequent transfer to permissive cells
Retrovirology
May 21, 2022
Burnie J, Persaud AT, Thaya L, Liu Q, Miao H, Grabinsky S, Norouzi V, Lusso P, Tang VA, Guzzo C.
were diluted in TBST and incubated for 1 h at room temperature:
goat
anti-
human
(
1:10 000
;
Sigma
, Cat#
AP112P
),
goat
anti-
mouse
(
1:10 000
;
Enzo
Life Science, Cat#
BML-SA204-0100
).
Membranes
were washed and developed
(12)
Autoantibodies against NCAM1 from patients with schizophrenia cause schizophrenia-related behavior and changes in synapses in mice
Cell reports. Medicine
April 19, 2022
Shiwaku H, Katayama S, Kondo K, Nakano Y, Tanaka H, Yoshioka Y, Fujita K, Tamaki H, Takebayashi H, Terasaki O,
et al
.
USA); HRP-linked anti-mouse IgG (
1:3000
; NA931, Cytiva, USA); and HRP-linked anti-human IgG (
1:3000
;
AP112P
,
Merck Millipore
, Burlington, MA, USA). Proteins were detected using ECL Prime
Western Blotting Detection
(13)
High-throughput identification of autoantibodies that target the human exoproteome
Cell Reports Methods
February 28, 2022
Eric Y. Wang et al.
Biotin anti-human IgG Fc
antibody,
BioLegend
,Cat#
409307
; RRID:AB_11150771 Goat anti-human IgG HRP,
Millipore Sigma
,Cat#
AP112P
; RRID:AB_90720
Mouse anti-human IgG1 Fc HRP
,
Southern Biotech
,Cat#
9054-05
; RRID:AB_2796627
Mouse anti-human IgG2 Fc HRP
(14)
Combination Treatment with the Vimentin-Targeting Antibody hzVSF and Tenofovir Suppresses Woodchuck Hepatitis Virus Infection in Woodchucks
Cells
September 5, 2021
Kyle E. Korolowicz, Manasa Suresh, Bin Li, Xu Huang, Changsuek Yon, Bhaskar V. Kallakury, Kyoung-pil Lee, Sungman Park, Yoon-Won Kim, Stephan Menne
1:1000
dilution) and HRP-conjugated anti-human IgG antibody (
Sigma-Aldrich
, St. Louis, MO, USA; cat. no.
AP112P
;
1:1000
dilution). Dosimetry was applied to compare
VIM
signals normalized to the housekeeping protein
(15)
Humanized anti-DEspR IgG4
S228P
antibody increases overall survival in a pancreatic cancer stem cell-xenograft peritoneal carcinomatosis rat
nu/nu
model
BMC cancer
April 14, 2021
Gromisch CM, Tan GLA, Pasion KA, Moran AM, Gromisch MS, Grinstaff MW, Carr FJ, Herrera VLM, Ruiz-Opazo N.
(25μg/ml),
anti-ADAR1
(
1:2000
), anti-Mcl-1 (
Santa Cruz
,
sc-12
,756,
1:200
), anti-human IgG HRP (
Sigma
AP112P
,
1:10000
), anti-rabbit IgG HRP (
Abcam
,
ab6721
,
1:10000
), and anti-mouse IgG HRP (Abcam,
ab6721
,
1:10000
).
(16)
Personalized immunoglobulin aptamers for detection of multiple myeloma minimal residual disease in serum
Communications Biology
December 1, 2020
Claudia Tapia-Alveal, Timothy R. Olsen, Tilla S. Worgall
and Genentech, respectively. Polyclonal human IgG (cat # 12511) and goat anti-human IgG-HRP (cat #
AP112P
) were purchased from
Sigma-Aldrich
. Patient samples De-identified serum samples from patients with
(17)
Human Heavy Chain Antibody Genes Elicited in Thai Dengue Patients during DENV2 Secondary Infection
Japanese Journal of Infectious Diseases
March 24, 2020
Nipa Thammasonthijarern, Wilarat Puangmanee, Pimolpachr Sriburin, Subenya Injampa, Supawat Chatchen, Weerapong Phumirattanaprapin, Chonlatip Pipattanaboon, Pongrama Ramasoota, Pannamthip Pitaksajjakul
1:3
) for 2 h. After washing, the
membranes
were incubated with HRP-conjugated
goat
anti-
human
IgG (
AP112P
,
Merck Millipore
, Temecula, CA, USA) for 1 h. The interaction signal was developed with ECL Prime chemiluminescent
(18)
Seroprevalence in Bats and Detection of Borrelia burgdorferi in Bat Ectoparasites
Microorganisms
March 20, 2020
Arinjay Banerjee, Kaushal Baid, Taylor Byron, Alyssa Yip, Caleb Ryan, Prasobh Raveendran Thampy, Hugh Broders, Paul Faure, Karen Mossman
1:10,000
dilution of goat anti-human IgG-HRP conjugate (
Millipore
, Burlington, MA, USA; catalogue number:
AP112P
) and strips that had been incubated with bat sera were incubated with a
1:5000
dilution of
goat
anti-bat
(19)
Fc Characteristics Mediate Selective Placental Transfer of IgG in HIV-Infected Women
Cell
June 13, 2019
David R. Martinez, Youyi Fong, Shuk Hang Li, Fang Yang, Madeleine F. Jennewein, Joshua A. Weiner, Erin A. Harrell, Jesse F. Mangold, Ria Goswami, George R. Seage,
et al.
AB_1500566
Normal Human serum
,
Sigma-Aldrich
,Cat# S1-M Goat anti-human IgG HRP,
Sigma-Aldrich
,Cat#
AP112P
; RRID: AB_90720 Biological Samples,Biological Samples,Biological Samples Anti-HepB Surface Antigen
(20)
Ubiquitin Chain Enrichment Middle-Down Mass Spectrometry (UbiChEM-MS) Reveals Cell-Cycle Dependent Formation of Lys11/Lys48 Branched Ubiquitin Chains
Journal of Proteome Research
September 1, 2017
Ambar S. J. B. Rana, Ying Ge, Eric R. Strieter
HRP conjugate (
EMD Millipore
;
12–349
), and
Goat
antihuman IgG antibody, HRP conjugate (EMDMillipore;
AP112P
). Methods Author Manuscript Asynchronous
Cell Culture
—
HEK293 cells
were grown in media containing
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Goat Anti-Human IgG Antibody, HRP conjugate from MilliporeSigma
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
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