Fig 1: Lycorine treatment inhibits T cell infiltration and function in cardiac allografts. On day 7 posttransplant, allograft‐infiltrated leukocytes were isolated from PBS‐treated mice (Vehicle) or lycorine‐treated mice (5 mg kg−1, b.i.d. Lycorine) were isolated and analyzed by flow cytometry. A) Counts of allograft‐infiltrated cells and CD45+ cells in each group (n = 6). B) Numbers of allograft‐infiltrated CD4+ T cells, CD8+ T cells, B cells and NK cells (n = 6). C) The expression of membrane CD69, CD25, OX40, and GITR on the surface of allograft‐infiltrated CD4+ T cells and CD8+ T cells was measured (n = 5). Values for mean fluorescence intensity (MFI) are shown. D) Bar graphs depict MFI values for PD1, CTLA4, LAG3, and TIM3 on T‐cell subsets (n = 5). E) Allograft‐infiltrated leukocytes were stimulated with a Cell Stimulation Cocktail (plus protein transport inhibitors) and intracellular staining was performed. Percentages of IFN‐γ + and TNF‐α + T‐cell subsets are shown (n = 5). F) Levels of CD107a expression on allograft‐infiltrating CD4+ T cells and CD8+ T cells from each group (n = 5). The graphs show the means ± SEM of three independent experiments. *P < 0.05, and **P < 0.01, Student's t‐test.