Fig 1: Enhanced cleaved caspase 3, MEK, ERK, STAT3, and suppressed Arg-1 signaling in inflamed extracellular vesicles incubated macrophages. THP-1 cells were exposed to inflamed, subcutaneous or noninflamed MSCs or extracellular vesicles for 72 h in macrophage differentiation conditions before protein harvest and western blot analysis. (a) The levels of cleaved caspase 3, pMEK, MEK, pERK, ERK, STAT3, and ARG-1 after MSCs treatment were determined by immunoblotting. (b) The levels of cleaved caspase 3, pMEK, MEK, pERK, ERK, STAT3, and ARG-1 after extracellular vesicles treatment were determined by immunoblotting. Relative band intensities were quantified from five individual experiments and measured by densitometry analysis. Beta-actin was used as a loading control. Phosphorylated proteins were further normalized by total protein values. Mes, diseased mesentery; MSC, mesenchymal stem cells; Subq, subcutaneous; WO, without treatment. n = 3 patients. *p < 0.05.