Fig 2: RNA-seq of spontaneously differentiated hiPSCs. (A) Hierarchical clustering heat map of gene expression by hiPSCs cultured on the different pECM hydrogels and matrigel relative to undifferentiated hiPSCs. Relative expression (z-score) is displayed in colors, in which orange indicates upregulation and blue indicates downregulation. (B,C) Principal component analysis (PCA) of the top 2000 variable genes. (B) A comparison between cells cultured on all pECM hydrogels, matrigel, and undifferentiated hiPSCs control. (C) A comparison between cells cultured on the pECM hydrogels. (D) Heat map of relative gene expression (z-score) by hiPSCs cultured on the different pECM hydrogels. Maroon indicates upregulation and white indicates downregulation. (E,G) Expression of selected genes relative to undifferentiated hiPSCs. (E) Genes associated with pluripotency: NKX 1-2, NANOG, SOX-2. (F) Genes associated with ECM and ECM remodeling: Fibronectin 1, MMP1, MMP2, MMP9, NKX 2-3, NKX 3-2. (G) Specific tissue differentiation: MYH6, MYH7, TNNT3, PLN, CD34, VEGFB, VEGFD, PDX1, LAMA5, and LAMA6.