Fig 1: IHC of the co-expression of key molecules other than ASCL1 in SCLC samples. Serial section immunostaining for NEUROD1 (A) and YAP1 (B): SCLC cell nuclei were positive for NEUROD1 (A), and the cytoplasm was positive for YAP1 (B). Serial section immunostaining for NEUROD1 (C) and POU2F31 (D): SCLC cell nuclei were positive for NEUROD1 (C), and the cytoplasm was positive for POU2F3 (D). The cytoplasm of SCLC cells was positive for YAP1 (E) and POU2F3 (F). Scale bar = 100 µm.
Fig 2: Example of the positive control of four key molecules by WB and IHC in SCLC samples. (A) Example of the positive control of the four key molecules by WB in SCLC cell lines. ASCL1 was strongly expressed in H209 cells. NeuroD1 was strongly expressed in H82 cells. Pou2F3 was strongly expressed in H526 cells and weakly expressed in H82 and SBC3 cells. YAP1 was strongly expressed in SBC3 cells. ß-actin served as an internal control. (B) Example of the positive control of the four key molecules in xenotransplanted tumor tissues from the four cell lines in mice by IHC. ASCL1 staining was found in tumor cell nuclei of H209 cells. NeuroD1 staining was found in the tumor cell nuclei of H82 cells. Pou2F3 staining with a diffuse cytoplasmic pattern was found in H82, H526, and SBC3 cells. The Pou2F3 staining intensity was weak in H82 and SBC3 cells and strong in H526 cells. YAP1 was stained with a membranous pattern in SBC3 cells. Scale bar = 50 µm.
Fig 3: IHC of the co-expression of ASCL1 and three key molecules in SCLC samples: Serial section immunostaining for ASCL1 (A) and NEUROD1 (B). SCLC cell nuclei were positive for ASCL1 (A) and NEUROD1 (B). Serial section immunostaining for ASCL1 (C) and YAP1 (D). SCLC cell nuclei were positive for ASCL1 (C), and the cytoplasm was positive for YAP1 (D). SCLC cell nuclei were positive for ASCL1 (E), and the cytoplasm was positive for POU2F3 (F). Scale bar = 100 µm.
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