Fig 1: The receptors and signaling pathways that are activated in NGF-induced BMSCs in vitro and in vivo. (a-d) qRT-PCR was to determine the expression levels of the ACAN, SOX9, COL2A1 and COL1A1 genes in the repaired model after 4, 8 and 12 weeks. (e) Western blots was used to analyze the protein expression levels of Col1A1 and COL2A1. (f and g) Western blots was used to analyze the protein expression levels of the NGF receptors TrkA and p75 in vitro (f) and in vivo (g). (h and i) Western blots was used to analyze the protein expression of proteins in the PI3K/AKT signaling pathway, including PI3K, AKT and p-AKT, in vitro (h) and in vivo (i). (j and k) Western blots was used to analyze the expression of proteins in the ERK/MAPK signaling pathway, including ERK, p-ERK, P38 and p-P38, in vitro (j) and in vivo (k). (l) Schematic description of the relevant signaling pathways that were activated by NGF. The values are means±S.D., n=10 joints; bars with different letters are significantly different from each other at P<0.05