Fig 1: Identification of specific molecules in Il18-/- mice that respond to acute stress.A The heatmap (left) and volcano map (right) generated from RNA sequencing are shown. (C; stressed Il18+/+ mice, KO; stressed Il18-/- mice). B The reverse transcription quantitative polymerase chain reaction results are shown. The molecules were extracted from RNA sequencing as shown in Table 1. C The protein expression of gamma-aminobutyric acid type A receptor subunit rho1 (GABRR1), heat shock transcription factor 5 (HSF5), and transthyretin (TTR) is shown. D The mRNA, protein, and serum levels of TTR/Ttr are shown. *p < 0.05, **p < 0.01, ***p < 0.001. (A; n = 3, B–D; n = 6, enzyme-linked immunosorbent assay n = 12 per group).