Fig 1: PAH catalyzes Phe and regulates PKM2 activity in T-ALL cells. a,b) Western blot analyses (a) and quantification (b) of PAH protein levels in Jurkat cells after PAH knockdown with indicated treatment (n = 3 independent experiments). c,d) Quantification of gas chromatography mass spectrometry for the cellular Phe levels (c) and Tyr levels (d) in Jurkat cells with indicated treatment (n = 3 independent experiments). e–g) Relative intracellular pyruvate kinase (e), ROS level (f), and apoptotic cells (g) in Jurkat cells with indicated treatment (n = 3 independent experiments). Data represent mean ± s.d. Two-tailed Student's t-tests were used to assess statistical significance. *p < 0.05, **p < 0.01, ***p < 0.001. Repeated measures one-way analysis of variance (ANOVA) followed by Dunnett's test for multiple comparisons, ? p < 0.05, ?? p < 0.01, ??? p < 0.001.