Fig 1: Perilipin-2 mRNA expression is increased in hPod exposed to active disease FSGS plasmas. hPod differentiated in T25 culture flasks (Corning) were exposed to FSGS plasmas. Active disease plasmas rec1A and nat1A increased PLIN2 mRNA expression compared to healthy plasma, whereas paired remission plasmas did not. Notably, active disease plasma rec8, which did not induce granularity, also did not increase PLIN2 mRNA expression. PLIN2 mRNA expression of two replicate experiments and their means relative to healthy plasma are depicted. *** p < 0.001 compared to healthy plasma. healthy: pooled plasma from five healthy donors; nat: patient with FSGS in the native kidneys; rec: patient with recurrent FSGS.