Fig 1: Regulation of Purine Degradation Pathway. (A) Representative Western blots are shown. Each lane represents a biological replicate. GAPDH is used as a loading control. Two isoforms of 5'-nucleotidase (Nt5c2 and NT5c3) are found to be less abundant in TAC rats compared to control while one (NT5c1a) is unchanged between TAC and control. The AMP deaminase AMPD3 is upregulated in TAC compared to control. (B) Using ImageJ to quantify the Western blots, there is a 42% and 66% decrease in NT5c2 and NT5c3, respectively, and a 121% increase in AMPD3 protein abundance in the TAC rats compared to control (error bar: SEM; NT5C3 P-value: 0.009; NT5C2 P-value: 0.05; sham n = 8; TAC n = 8).
Supplier Page from Abcam for Anti-PN-I antibody