Fig 1: Western blot of TLR pathway and platelet-related markers of the submandibular gland tissue of NOD mice. Submandibular gland was obtained at the age of 13 weeks, after intervention of CA-4948/Resiquimod/saline for 5 weeks. C: control group treated with saline; I: inhibitor group (treated with CA-4948); A: activator group (treated with Resiquimod). Compared with saline control group, Resiquimod induced significantly increased expression of the TLR7 signaling pathway molecules, interleukin (IL)-1ß, IL-8, and Megakaryocyte Colony Stimulating Factor (MK-CSF), and decreased expression of TPO in the submandibular glands. While CA-4948 group showed opposite expression trends of these molecules (*p < 0.05, **p < 0.01, ***p < 0.001; ns, not significant).
Fig 2: Hematoxylin and eosin-stained sections and Immunohistochemical (IHC) stained sections of the submandibular gland tissue of NOD mice. Submandibular gland was obtained at the age of 13 weeks, 5 weeks after intervention of CA-4948/Resiquimod/saline. (A) Infiltration of scattered lymphocytes was present in interstitial tissue (original magnification × 100). (B) IHC of Interleukin (IL)-1ß (original magnification × 400). (C) IHC of IL-8 (original magnification × 400). (D) IHC of Megakaryocyte Colony Stimulating Factor (MK-CSF) (original magnification × 400). (E) IHC of thrombopoietin (TPO) (original magnification × 400). Expression levels of IL-1ß, IL-8, and MK-CSF were stronger in the Resiquimod-treated group than in the saline control. However, decreased expression of IL-1ß, IL-8, and MK-CSF and increased expression of TPO in the CA-4948 group were observed compared to that in the control group.
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