Fig 1: Knockdown of Eps8 influences EMT markers in HeLa and SiHa cells. (A) Western blotting confirmed the upregulation of E-cadherin and downregulation of Vimentin and snail at the protein level in HeLa and SiHa cells following Eps8 knockdown. GAPDH was used as a loading control. (B) Reverse transcription-quantitative polymerase chain reaction revealed that Eps8 knockdown in HeLa and SiHa cells increased the mRNA expression of E-cadherin, but decreased the mRNA expression levels of vimentin and snail expression. The data are presented as the mean ± standard deviation of triplicate experiments (**P<0.01, *P<0.05 compared with the control shRNA). Esp8, epidermal growth factor receptor kinase substrate 8; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; sh, short hairpin.
Fig 2: Effects of Eps8 knockdown on the migration and invasion capacity of cervical cancer cells. (A) Transwell migration and invasion assays were performed in Eps8-shRNA transfected HeLa and SiHa cells. Following incubation for 24 h, migrated/invading cells were counted under a microscope and images were captured. Figures are representative of multiple experiments. (B) The data were quantified and are presented as the mean ± standard deviation of three independent experiments (**P<0.01 compared with the control shRNA). Esp8, epidermal growth factor receptor kinase substrate 8; sh, short hairpin.
Fig 3: Expression levels of Eps8 and EMT markers in cervical cancer samples. (A) Immunohistochemical staining for Eps8 and the EMT markers, E-cadherin and Vimentin, in NC, LSIL, HSIL and SCC tissues (magnification, ×400). (B) The expression levels of Eps8, E-cadherin and Vimentin were categorized as negative, low or high expression in the normal cervix, LSIL, HSIL, and SCC samples. Esp8, epidermal growth factor receptor kinase substrate 8; EMT, epithelial-mesenchymal transition; NC, normal cervical epithelia; LSIL, low-grade squamous intraepithelial lesion; HSIL, high-grade squamous intraepithelial lesion; SCC, squamous cell carcinoma.
Supplier Page from Abcam for Anti-EPS8 antibody