Fig 1: Prdm16 is a potential target of miR-188 in the regulation of the aging-associated metabolic phenotype. (a) Schematic of the sequence that miR-188 targets the WT or mutated 3' UTR of Prdm16 mRNA. (b) The luciferase activity of Prdm16 WT or mutated 3' UTR reporter plasmids co-transfected with miR-188 mimics or miR-NC mimics in HEK293 cells as indicated. (c, d) The mRNA levels of Prdm16 in primary cultured brown adipocytes transfected with miR-188 mimics or miR-NC mimics. (e, f) The protein levels of PRDM16 in primary cultured brown adipocytes transfected with miR-188 mimics or miR-NC mimics. (g, h) The protein levels of PRDM16 and UCP1 in BAT of 18-month old miR-188 knockout mice and miR-188 Tg mice as indicated. Data are shown as means ± SEM (n = 5–7 in g, h), the cell experiments were repeated for at least three times. Statistical significance was calculated by Student's t test or two-way ANOVA, ***p < .001
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