Fig 1: TAN alleviated LPS‐induced cell viability decrease and inflammatory response by promoting VLDLR expression. (a) Western blot was used to detect the transfection efficiency of siVLDLR. (b) Gray value analysis of Western blot results. (c) qRT‐PCR was used to detect the transfection efficiency of siVLDLR. (d) MTT was used to detect the cell viability of FHC cells treated with or without siVLDLR, LPS and TAN. (e–g) ELISA was used to measure the content of IL‐6, IL‐8, IL‐1β in FHC cells treated with or without siVLDLR, LPS and TAN. (h–j) qRT‐PCR was used to identify the expressions of IL‐6, IL‐8, IL‐1β in FHC cells treated with or without siVLDLR, LPS and TAN. GAPDH severs as an internal reference. & Vs. siNC, * vs. control, # vs. LPS + vector, ^ vs. LPS + TAN + vector; && P < .01, *** P < .001, ### P < .001, ^^^ P < .001. qRT‐PCR, quantitative reverse transcription polymerase chain reaction; MTT, 3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide; TAN, tanshinol; IL, interleukin; VLDLR, low density lipoprotein receptor; ELISA, enzyme‐linked immunosorbent assays; FHC, normal colorectal mucosal cell line
Fig 2: TAN alleviated DSS-induced UC and VLDLR expression decrease. (a) The survival rate of mice was recorded. (b) The colons length of mice was measured. (c) H&E staining of colonic tissue in mice. Scale: 200 µm; magnification: ×40. (d–f) The levels of TNF-a, IL-1ß, and IL-6 in colon tissue were detected by ELISA. (g) The expression of VLDLR of mice colonic tissues was determined by Western blot. (h) Gray value analysis of Western blot results. GAPDH severs as an internal reference. * Vs. control, # vs. model. # P < .05; ## P < .01, *** P < .001, ### P < .001. UC, ulcerative colitis; ELISA, enzyme-linked immunosorbent assays; VLDLR, low density lipoprotein receptor; H&E, hematoxylin–eosin staining
Fig 3: TAN alleviated LPS-induced FHC cell apoptosis by promoting VLDLR expression. (a) Flow cytometry was used to detect the cell apoptosis of FHC cells treated with or without siVLDLR, LPS and TAN. (b) The expressions of Bax, Bcl-2, cleaved caspase-3 and cleaved caspased-9 in FHC cells treated with or without siVLDLR, LPS and TAN was detected by Western blot. (c–f) Gray value analysis of Western blot results. GAPDH severs as an internal reference. * Vs. control; # vs. LPS + vector; ^ vs. LPS + TAN + vector; *** P < .001, ### P < .001, ^^^ P < .001. TAN, tanshinol; LPS, lipopolysaccharide; Bax, Bcl-2 associated X; Bcl-2, B-cell lymphoma-2; VLDLR, low density lipoprotein receptor; FHC, normal colorectal mucosal cell line
Fig 4: TAN alleviated LPS-induced FHC cells apoptosis. (a) Flow cytometry was used to detect the cell apoptosis of FHC cells treated with or without LPS and TAN. (b, g) The expressions of Bax, Bcl-2, cleaved caspase-3, cleaved caspased-9 and VLDLR in of FHC cells treated with or without LPS and TAN was detected by Western blot. (c–f, h) Gray value analysis of Western blot results. (i) qRT-PCR was used to detect the expressions of VLDLR in FHC cells treated with or without LPS and TAN. GAPDH severs as an internal reference.* Vs. control, # vs. LPS; *** P < .001, ### P < 0.001. TAN, tanshinol; LPS, lipopolysaccharide; Bax, Bcl-2 associated X; Bcl-2, B-cell lymphoma-2; FHC, normal colorectal mucosal cell line
Fig 5: VLDLR was low-expressed in UC tissues. (a) Immunohistochemistry was used to detect VLDLR expression in colonic mucosal tissues of UC patients and normal controls. Scales: 50 and 15 µm; magnifications: ×100 and ×200. (b) The expression of VLDLR in colonic mucosal tissues of UC patients and normal controls was detected by qRT-PCR. GAPDH severs as an internal reference. (c) The expression of VLDLR in colonic mucosal tissues of UC patients and normal controls was detected by Western blot. (d) Gray value analysis of Western blot results. GAPDH severs as an internal reference. * Vs. Normal; ***P < .001. UC, ulcerative colitis; qRT-PCR, quantitative reverse transcription polymerase chain reaction; VLDLR, low density lipoprotein receptor
Supplier Page from Abcam for Anti-VLDL Receptor/VLDL-R antibody