Fig 1: Time-dependent changes in repulsive proteins expression after thoracic spinal cord injury. Western blots of Neurocan (A), Phosphacan (B), Nogo-A (C), MAG (D), OMgp (E) and RGMa (F) and respective ratio against the endogenous protein GAPDH at the lumbosacral spinal cord in spinal intact animals (control) and after SCT. Total Neurocan (A) expression did not show any significant differences between controls and SCT animals, but Neurocan-250 significantly increased 7 dpi and Neurocan-160 increased significantly 28 dpi. Total Phosphacan (B) and Nogo-A (C) expression significantly increased 7 dpi (days post-injury), decreasing at 28 dpi. Expression of MAG (D), OMgp (E) and RGMa (F) was not changed by the thoracic lesion. Graphs represent mean ± SD and p < 0.05 was considered statistically significant. * p = 0.05; *** p = 0.001; one-way ANOVA followed by Tukey’s multiple comparison test.
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