Fig 1: Transcripts encoding ionotropic glutamate receptors are expressed in mouse blastocysts and oocytes. Subunits of ionotropic glutamate receptors are listed in the table on the left. The most frequently used common names of subunits are given in parentheses. Mouse gene symbols are used (human gene symbols are the same but written with all letters capitalized, e.g., GRIA1). In transcripts which were consistently expressed in both blastocysts and oocytes, fold regulation values (“+" means upregulation and “-" means downregulation in blastocysts compared to oocytes) and corresponding P-values are shown. Transcripts were detected by reverse transcription-polymerase chain reaction (RT-PCR) and representative agarose gels with separated PCR products are shown in the panels on the right. Lanes: Gria1, Glutamate receptor, ionotropic, AMPA1; Gria2, Glutamate receptor, ionotropic, AMPA2; Gria3, Glutamate receptor, ionotropic, AMPA3; Gria4, Glutamate receptor, ionotropic, AMPA4; Grik1, Glutamate receptor, ionotropic, kainate 1; Grik2, Glutamate receptor, ionotropic, kainate 2; Grik3, Glutamate receptor, ionotropic, kainate 3; Grik4, Glutamate receptor, ionotropic, kainate 4; Grik5, Glutamate receptor, ionotropic, kainate 5; Grin1, Glutamate receptor, ionotropic, NMDA1; Grin3a, Glutamate receptor, ionotropic, NMDA3A; Grin3b, Glutamate receptor, ionotropic, NMDA3B; Grin2a, Glutamate receptor, ionotropic, NMDA2A; Grin2b, Glutamate receptor, ionotropic, NMDA2B; Grin2c, Glutamate receptor, ionotropic, NMDA2C; Grin2d, Glutamate receptor, ionotropic, NMDA2D; MW, molecular weight markers; (A) positive control tissue; (B) ovulated oocytes; (C) blastocysts. The MWs in base pairs (bp) are indicated to the right of the panels. *Primers for Grik3, Grin2d, Grin3a, and Grin3b subunits were not included in the Mouse GABA & Glutamate RT2 profiler PCR array, and commercial primer sets from Qiagen were used (see Materials and methods). Delta receptors (formed from delta 1 and delta 2 subunits), classified by sequence homology as ionotropic glutamate receptors, do not bind glutamate, and were not investigated in this study.