Purified anti-Pax-2 901001 from BioLegend

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International Journal of Molecular Sciences
January 28 2021

Fig 1: Pax2 expression in the pallium and subpallium of intact juvenile chum salmon, Oncorhynchus keta. (A) General view of the Pax2 expression pattern in the telencephalon of intact juvenile chum salmon where the boundaries between the pallial and subpallial regions are indicated by the red dashed line, Pax2+ cells along the border are indicated by thin red arrows; the pictograms indicate the areas of the brain shown in Figures (B–D) and (E–G). (C) Pax2 expression in DD, clusters of Pax2-expressing cells (red arrow) in PVZ (black inset) and nuclei (blue arrow, shown in red inset); the aggregation of nuclei is outlined by the red oval, and in black ovals there are aggregations of immunonegative cells in PZ, white arrows indicate weakly labeled cells; (C) patterns of Pax2 expression in DM; immunolabeling of Pax2 was detected in nuclei (shown by yellow arrows), which form extended regions in PVZ and SVZ (black inset). the granular pattern of Pax2 expression predominated in PZ, and the red dotted line indicates the radial direction of neuroblasts’ migration from constitutive neurogenic niches (CNN) (red inset) containing clusters of Pax2– (encircled by the white dotted line) and Pax2+ cells; (D) morphological heterogeneity of Pax2+ cells in DL, where the black rectangle outlines nuclei labeled with Pax2; Pax2+ cells were found in the basal part of PVZ and SVZ, both cellular (in the dashed rectangle) and nuclear expression of Pax2 (in the red ovals); (E) in PVZ of the dorsal subpallial region (VD), Pax2+ cells (red arrows) had a surface tangential orientation (red inset) and in SVZ and PZ there were moderately (in the dotted square) and weakly labeled Pax2 cells (in the oval), migrating cells (thin red arrows) and nuclei (yellow arrows) that form morphogenetic fields; (F) In VL, individual Pax2+ nuclei were encountered in PVZ (black inset); in SVZ, individual intensely labeled clusters of cells (red inset) were identified. In VL, local Pax2– cell clusters were identified in combination with dense clusters of Pax2+ cells (in dotted rectangle ); (G) intensely labeled clusters of Pax2+ cells (black inset) were detected in PVZ and the aggregation of immunonegative basophilic cells was found in PZ (indicated by pink arrows, red inset); the red dotted line indicates the direction of neuroblast migration and in the red oval there is a morphogenetic field with weak expression of Pax2 and neuroblasts. Immunohistochemical labeling of the protein product of the Pax2 transcription factor. Scale bars: (A) 500 µm; (B,C,F) 100 µm; (D,E) 50 µm. (H) Ratio of Pax2+/Pax2–cells in the pallium and subpallium of intact chum salmon (n = 5 in each group, #—significant intergroup differences (p < 0.05)). One-way ANOVA. (J) Comparative densitometric analysis of Pax2+ cells; significant intergroup differences # (p < 0.05) between groups of intensively, moderately, weakly labeled, and immunonegative cells (n = 5 in each group). One-way ANOVA. Intensity of Pax2 labeling, UOD. (K) Ratio of the number of Pax2+/Pax2—cells in the pallium and subpallium zones at 3 days after the injury to the telencephalon of juvenile chum salmon; #—significant intergroup differences (n = 5 in each group, #—significant intergroup differences (p < 0.05). One-way ANOVA.
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Scientific reports
November 25 2022

Fig 2: Expression of renal progenitor markers in renal progenitors derived from proximal tubule cell induced pluripotent stem cells (PTC iPSC) compared to renal progenitors derived from tail tip fibroblast induced pluripotent stem cells (TTF iPSC). RT-qPCR shows that Pax2, Gata3 and Jag1 expression are higher in renal progenitors derived from PTC iPSC csmpared to renal progenitors derived from TTF iPSC. Y-axis gene expression is relative to E13.5 mouse embryonic kidney which is set at 1.0. Box and whiskers indicate quartiles and maximum/minimum values respectively, with central line showing the median. Asterisk (*) indicates that expression was significantly different (p < 0.05) from progenitors derived from PTC iPSCs, as determined by t test. n = 4 PTC iPSC lines and 4 TTF iPSC lines, 3 replicates per line.
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Scientific reports
July 03 2017

Fig 3: Generation of PAX2-deficient human iPS cells. (A) PAX2 staining during nephron formation from the parental iPS clone. PAX2 is expressed in the renal vesicles (left panel: green), NCAM+ S-shaped bodies (middle panel: green), and E-cadherin (ECAD)+ distal renal tubules (right panel: green). LHX1 (left panel: red) is expressed in a portion of renal vesicles, and podocalyxin (PODXL; right panel: blue) is expressed in glomeruli. Scale bars: 50 µm. (B) Strategy for targeting the human PAX2 gene. The TurboGFP and RFP cassettes are inserted into the PAX2 locus. The RFP and Puro cassettes are sandwiched by loxP (arrowheads) and insulator sequences (hexagons). A, ApaLI; H, HindIII. (C) Southern blot analysis of wild-type, heterozygous (+/-), and homozygous (-/-) clones. Genomic DNAs were digested with Hind III (upper panel) or ApaLI (lower panel) and hybridised with the indicated probes. The positions of the probes are indicated in (B). *Non-specific bands. (D) PCR screening of homologous recombinants. The positions of the primers are indicated in (B). (E) Western blot analysis of PAX2 in nephron progenitors derived from iPS clones.
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Scientific reports
July 03 2017

Fig 4: Successful deletion of PAX2 in renal tubules derived from iPS cells. (A) Hematoxylin–eosin (HE) staining of kidney tissues induced from wild-type (+/+), heterozygous (+/-), and homozygous (-/-) clones. Arrowheads: glomeruli; arrows: renal tubules. (B) PAX2 (red) and GFP (green) staining of kidney tissues induced from the three genotypes. Rightmost columns: neighbouring sections were stained with markers for renal tubules (E-cadherin: ECAD) and glomerular podocytes (podocalyxin: PODXL). (C) Nephron formation from a mixed colony of iPS cells. Nephron epithelia are composed of PAX2+ wild-type and GFP+ mutant cells. Scale bars: 50 µm.
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Scientific reports
July 03 2017

Fig 5: Nephron progenitors are generated from PAX2-null iPS cells. (A) FACS analysis of tissues induced from wild-type (+/+) and PAX2-null (-/-) iPS cells. The percentages of the ITGA8+/PDGFRA- nephron progenitor fraction from three independent induction experiments are determined (mean ± standard error). (B) Nephron progenitor markers are more abundantly expressed in the ITGA8+/PDGFRA- fraction (open bars) than in the ITGA8-/PDGFRA- fraction (closed bars) induced from the wild-type clone. Data represent mean ± standard error of three independent induction experiments. *p < 0.05, **p < 0.01. (C) Tubulogenesis is observed when the ITGA8+/PDGFRA- fraction is co-cultured with spinal cord for 9 days. SP: spinal cord. Scale bars: 200 µm. (D) Expression levels of nephron progenitor markers are unaltered, except for PAX2. qPCR analyses of sorted ITGA8+/PDGFRA- fractions from two wild-type clones (open bars) and two PAX2-null clones (closed bars) are shown (mean ± standard error). **p < 0.01.

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Purified anti-Pax-2

BioLegend's Purified anti-Pax-2 is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Immunohistochemistry, Immunohistochemistry - fixed, Immunoprecipitation, and Western Blot. The Purified anti-Pax-2 Antibody was generated using PAX2 as the antigen and it reacts with Homo Sapiens, and Human.

Description

Purified anti-Pax-2 [Poly19010]

Product Specs
ItemPurified anti-Pax-2
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number901001
Quantity200 µL
ApplicationsImmunohistochemistry (IHC)-P, Western Blot (WB), Immunoprecipitation (IP)
Concentration1.00 mg/mL
Conjugate/TagUnconjugated
FormatPhosphate-buffered solution + 0.03% Thimerosal
ReactivityHuman
ClonePoly19010
HostRabbit
ImmunogenThis Pax-2 antibody was generated against a recombinant protein containing 22kD of the Pax-2 sequence corresponding to amino acids 188-385
NCBI Full Gene Namepaired box 2
NCBI Gene AliasesFSGS7, PAPRS, PAX-2
ClonalityPolyclonal
Brand/TrademarkPRB-276P-200
IsotypeIgG
TargetPax-2

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(1) Single-cell sequencing of individual retinal organoids reveals determinants of cell fate heterogeneitybioRxiv : the preprint server for biologyJune 3, 2023Tresenrider A, Sridhar A, Eldred KC, Cuschieri S, Hoffer D, Trapnell C, Reh TA.Antibody,Source,Catalog #,Dilution POU4F2,Santa Cruz Biotechnology,SC-6026,1:300 PAX2,BioLegend,901001,1:300 VSX2,Exalpha Biologicals,X1180P,1:300 VSX2,SantaCruz,Sc-365519,1:300 MAP2,Sigma,M9942,1:200 OTX2,ABCAM,21990,1:300 ELAVL3/4,Invitrogen,A-21271,1:100 RCVRN,Chemicon,AB5585,1:200

(2) Modeling kidney development, disease, and plasticity with clonal expandable nephron progenitor cells and nephron organoidsbioRxiv : the preprint server for biologyMay 25, 2023Huang B, Zeng Z, Li H, Li Z, Chen X, Guo J, Zhang CC, Schreiber ME, Vonk AC, Xiang T, et al.Monoclonal anti-WT1,Abcam,"Cat# ab89901, RRID: AB_2043201" Rabbit Polyclonal anti-PAX2,BioLegend,"Cat# 901001, RRID: AB_2565001" Rabbit Polyclonal anti-PAX8,Proteintech,"Cat# 10336-1-AP, RRID: AB_2236705" Mouse

(3) Functional genomics analysis identifies loss of HNF1B function as a cause of Mayer-Rokitansky-Küster-Hauser syndromeHuman molecular geneticsMarch 6, 2023Thomson E, Tran M, Robevska G, Ayers K, van der Bergen J, Gopalakrishnan Bhaskaran P, Haan E, Cereghini S, Vash-Margita A, Margetts M, et al.FOXL2 (1:100, Novus Biologicals, Colorado, USA, #NB100–1277), PAX2 (1:100, BioLegend, California, USA, #901001), PH3 (1:100, Abcam, United Kingdom, #5176), LAMA (1:100, Sigma, Missouri, USA, #L9393), VIM (1:200,

(4) Determining epigenetic memory in kidney proximal tubule cell derived induced pluripotent stem cells using a quadruple transgenic reprogrammable mouseScientific reportsNovember 25, 2022Khelifi G, Chow T, Whiteley J, Fort V, Humphreys BD, Hussein SMI, Rogers IM.(Thermo Fisher, PA5-27438), Six2 (ProteinTech, 11562-1-AP), Wt1 (Abcam, AB89901), Pax2 (Biolegend, 901001). Secondary antibodies: anti-rabbit IgG Cy5 and anti-mouse IgG 488. Teratoma formation assay Four

(5) Fadrozole-mediated sex reversal in the embryonic chicken gonad involves a PAX2 positive undifferentiated supporting cell stateFrontiers in Cell and Developmental BiologyNovember 10, 2022Martin A. Estermann, Craig A. Smithwith the primary antibody in 1% BSA in 1X PBS. Primary antibodies used: rabbit anti-PAX2 (Biolegend 901001, 1; 500), rabbit anti-DMRT1 (in house antibody; 1:2,000), rabbit anti-SOX9 (Millipore AB5535, 1:4,000),

(6) Modeling injury and repair in kidney organoids reveals that homologous recombination governs tubular intrinsic repairScience translational medicineMarch 2, 2022Gupta N, Matsumoto T, Hiratsuka K, Garcia Saiz E, Galichon P, Miyoshi T, Susa K, Tatsumoto N, Yamashita M, Morizane R.,Santa Cruz,Sc-13131,1:100 MEIS1,Active motif,39796,1:100 NG2,BD biosciences,554275,1:100 OCT2,Abcam,ab170871,1:200 PAX2,Biolegend,901001,1:500 PDGFR-β,R&D systems,AF385,1:200 PODXL,R&D systems,AF1658,1:500 PTEN,Cell signaling,9188S,1:100 SIX1,Cell signaling

(7) Ex vivo development of the entire mouse fetal reproductive tract by using microdissection and membrane-based organ culture techniquesDifferentiationJanuary 6, 2022Shuai Jia, Fei Zhaoat room temperature then with the primary antibody (anti-PAX2 antibody, 1:300; Biolegend, Catalog #: 901001. 1:300) in the blocking solution overnight at 4°C with rocking. Tissues were washed with PBST (1%Triton-100

(8) Spatial transcriptional mapping of the human nephrogenic programDevelopmental cellAugust 23, 2021Lindström NO, Sealfon R, Chen X, Parvez RK, Ransick A, De Sena Brandine G, Guo J, Hill B, Tran T, Kim AD, et al.,Santa Cruz,sc-19341 anti-Mafb,R&D,MAB3810 anti-Mafb,Santa Cruz,sc-10022 anti-Mecom,R&D,MAB75061 anti-Pappa2,R&D,AF1668 anti-Pax2,Biolegend,901001 anti-Pax8,Abcam,ab189249 anti-Podxl,R&D,AF1658 anti-Pou3f3,Thermo Scientific,PA5–64311 anti-Pou3f3,Novus Biologicals

(9) The embryonic ontogeny of the gonadal somatic cells in mice and monkeysCell reportsMay 4, 2021Sasaki K, Oguchi A, Cheng K, Murakawa Y, Okamoto I, Ohta H, Yabuta Y, Iwatani C, Tsuchiya H, Yamamoto T, et al.

(10) MicroRNAs mediate precise control of spinal interneuron populations to exert delicate sensory-to-motor outputseLifeMarch 31, 2021Chang SH, Su YC, Chang M, Chen JA.

(11) Human spinal GABA neurons alleviate spasticity and improve locomotion in rats with spinal cord injuryCell reportsMarch 23, 2021Gong C, Zheng X, Guo F, Wang Y, Zhang S, Chen J, Sun X, Shah SZA, Zheng Y, Li X, et al.

(12) Atoh7-independent specification of retinal ganglion cell identityScience advancesMarch 1, 2021Brodie-Kommit J, Clark BS, Shi Q, Shiau F, Kim DW, Langel J, Sheely C, Ruzycki PA, Fries M, Javed A, et al.(Molecular Probes, catalog nos. I21411 and I21411, RRID:AB_2314662) (1:250), Rabbit anti-Pax2 (BioLegend, catalog no. 901001, RRID:AB_2565001) (1:100), Mouse anti-Tuj1 (R&D Systems, catalog no. MAB1195, RRID:AB_357520) (1:200),

(13) Mechanical Brain Injury Increases Cells’ Production of Cystathionine β-Synthase and Glutamine Synthetase, but Reduces Pax2 Expression in the Telencephalon of Juvenile Chum Salmon, Oncorhynchus ketaInternational Journal of Molecular SciencesJanuary 28, 2021Evgeniya V. Pushchina, Eva I. Zharikova, Anatoly A. Varaksinsitu with primary rabbit anti-Pax2 polyclonal antibodies (Biolegend, San Diego, CA, USA; Catalog No. 901001; 1:300), rabbit anti-CBS polyclonal antibodies (GeneTex, Irvine, CA, USA; Catalog No. GTX124346; 1:300),

(14) Resolving the heterogeneity of diaphragmatic mesenchyme: a novel mouse model of congenital diaphragmatic herniaDisease Models & MechanismsJanuary 1, 2021Louise Cleal, Sophie L. McHaffie, Martin Lee, Nick Hastie, Ofelia M. Martínez-Estrada, You-Ying Chau1:1000), anti-Ki67 (Abcam, ab15580, 1:1000), anti-GFP (Abcam, ab5450, 1:1000), anti-PAX2 (Biolegend, 901001, 1:100), anti-MF20 (DSHB, Ab_2147781, 1:20), anti-VIM (Santa Cruz Biotechnology, sc-7557, 1:100), anti-CDH1

(15) PAX2 (+) Mesenchymal Origin of Gonadal Supporting Cells Is Conserved in BirdsFrontiers in cell and developmental biologyJanuary 1, 2021Estermann MA, Mariette MM, Moreau JLM, Combes AN, Smith CA.overnight at 4°C in 1% BSA in PBS. The following primary antibodies were used: rabbit anti-PAX2 (Biolegend 901001, 1;500), rabbit anti-DMRT1 (in house antibody; 1:2000), rabbit anti-AMH (Abexa ABX132175; 1:1000), rabbit

(16) A molecular and cellular analysis of human embryonic optic fissure closure related to the eye malformation colobomaDevelopmentDecember 15, 2020Aara Patel, Glenn Anderson, Gabriel L. Galea, Monika Balys, Jane C. Sowden1:300, Life Technologies, 402200; anti-PAX6, 1:1000, Covance-Biolegend 901301; anti-Pax2, 1:300, Biolegend 901001) Slides were washed in PBS (3×5 min). The secondary antibody (Alexa Fluor 594 goat anti-rabbit IgG or Alexa

(17) Insights into Gonadal Sex Differentiation Provided by Single-Cell Transcriptomics in the Chicken EmbryoCell reportsApril 7, 2020Estermann MA, Williams S, Hirst CE, Roly ZY, Serralbo O, Adhikari D, Powell D, Major AT, Smith CA.

(18) A Modular Differentiation System Maps Multiple Human Kidney Lineages from Pluripotent Stem CellsCell reportsApril 7, 2020Tsujimoto H, Kasahara T, Sueta SI, Araoka T, Sakamoto S, Okada C, Mae SI, Nakajima T, Okamoto N, Taura D, et al.

(19) Simultaneous Requirements for Hes1 in Retinal Neurogenesis and Optic Cup-Stalk Boundary MaintenanceThe Journal of neuroscience : the official journal of the Society for NeuroscienceFebruary 12, 2020Bosze B, Moon MS, Kageyama R, Brown NL.3 AB_141542 AB_2157171 Pou4f/Brn3 * Santa Cruz Goat SC 6026 1:50 IF 1, 3 AB_673441 Pax2 BioLegend Covance Rabbit 901001 PRB-276P 1:1000 IF 1, 2, 3 AB_2565001 AB_291611 Pax6 BioLegend Covance Rabbit 1:1000 IF 1,

(20) Recapitulating kidney development in vitro by priming and differentiating mouse embryonic stem cells in monolayersNPJ Regenerative medicineJanuary 1, 2020Chow T, Wong FTM, Monetti C, Nagy A, Cox B, Rogers IM.

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6/16/2021Anti-Pax2 Works Well for Immunofluorescence Staining Joanna CunananAnti-Pax2 (Rabbit, cat# 901001 from Biolegend) works really well on mouse embryonic kidneys. Positive staining in the nucleus is observed in early nephron progenitors, including cap mesenchyme cells, ... Read Review

International Journal of Molecular Sciences

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Purified anti-Pax-2 from BioLegend

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Purified anti-Pax-2 from BioLegend

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