Fig 1: Myo9b expression analysis in the central nervous system. Images show mouse tissues labeled for the indicated antigens: Myo9B, choline acetyltranserase (CHAT; which labels motor neurons), neurofilament medium (NF-M; which labels axons), and BRN3A (Pou4f1, which labels retinal ganglion cells), and counterstained with DAPI. (a, a') In the ventral region of the mouse spinal cord at P60, Myo9b in found in astrocytic processes and diffusely in the gray matter, but does not colocalize with CHAT-positive motor neurons. (b, b') In ventral roots, Myo9b is localized to the abaxonal Schwann cell cytoplasm, and also to axons. (c, c') Staining of optic nerves in transverse section shows Myo9b localization in glial cells but not axons. (d, d') Staining of mouse retina shows Myo9b expression in retinal ganglion cells (BRN3A-positive) and in photoreceptors. Bar is 9.32 µm in d'; 19.56 µm in a and a', 8.18 µm in b-c', and 31.55 µm in d. DAPI, 4,6-diamidino-2-phenylindole