FITC anti-BrdU 364104 from BioLegend

Product Specs
ItemFITC anti-BrdU
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number364104
Quantity100 tests
ApplicationsIntracellular Staining for Flow Cytometry (ICFC)
Conjugate/TagFITC
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
IsotypeIgG1, Kappa
Clone3D4
HostMouse
Antibody TypePrimary
ImmunogenIodouridine-conjugated ovalbumin
ClonalityMonoclonal
TargetBrdU

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BioLegend

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San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
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Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) RAGE inhibitor TTP488 (Azeliragon) suppresses metastasis in triple-negative breast cancernpj Breast CancerJuly 13, 2023Melinda Magna, Gyong Ha Hwang, Alec McIntosh, Katherine Drews-Elger, Masaru Takabatake, Adam Ikeda, Barbara J. Mera, Taekyoung Kwak, Philip Miller, Marc E. Lippman, et al.and stored at −20C for at least 2 h. Fixed cells were labeled with anti-BrdU-FITC (BioLegend Catalog# 364104) following manufacturer’s instructions, and counterstained with propidium iodide (p4170-25MG Sigma).

(2) Mulberroside A ameliorates CCl4‐induced liver fibrosis in mice via inhibiting pro‐inflammatory responseFood Science & NutritionJune 1, 2023Baozhang Shi, Jinqiang Qian, Hongyue Miao, Shuo Zhang, Yue Hu, Puqing Liu, Liping Xufollowed by incubation with FITC‐conjugated anti‐BrdU antibody according to instructions (Biolegend, Cat#364103). The flow cytometry assay was performed using a Canto II flow cytometer (BD Bioscience). The data were

(3) Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA ubiquitinationCell reportsApril 25, 2023Wang YC, Kelso AA, Karamafrooz A, Chen YH, Chen WK, Cheng CT, Qi Y, Gu L, Malkas L, Taglialatela A, et al.Cells were washed twice with PBS then stained with FITC conjugated anti-BrdU antibody (BioLegend, Cat# 364104) (1:100) at 37°C for 1 h. After incubation, cells were washed 3 times with PBS and DNA content were

(4) Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA polyubiquitinationbioRxiv : the preprint server for biologyFebruary 3, 2023Wang YC, Kelso AA, Karamafrooz A, Chen YH, Chen WK, Cheng CT, Qi Y, Gu L, Malkas L, Kung HJ, et al.Cells were washed twice with PBS then stained with FITC conjugated anti-BrdU antibody (BioLegend, Cat# 364104) (1:100) at 37°C for 1 h. After incubation, cells were washed 3 times with PBS and DNA content were

(5) Claspin-Dependent and -Independent Chk1 Activation by a Panel of Biological StressesBiomoleculesJanuary 7, 2023Hsiao HW, Yang CC, Masai H.Pharmingen. Anti-H2A.X phospho-S139 (613402), anti-Rat IgG Alexa Fluor 555 (405420), and FITC-anti-BrdU (364104) were purchased from Biolegend. RPA32 phospho-S4/S8 (A300-245A) and anti-MCM2 S53(A300-756A) were purchased

(6) Claspin is Required for Growth Recovery from Serum Starvation through Regulating the PI3K-PDK1-mTOR Pathway in Mammalian CellsMolecular and cellular biologyJanuary 1, 2023Yang CC, Masai H.cells were treated with FITC-conjugated anti-BrdU antibody (BD biosciences, 51-33284X or Biolegend, 364103) for 20 min at room temperature in the dark, and further incubated with propidium iodide (25 µg/mL)

(7) Zinc finger protein Zfp335 controls early T-cell development and survival through β-selection-dependent and -independent mechanismseLifeFebruary 3, 2022Wang X, Jiao A, Sun L, Li W, Yang B, Su Y, Ding R, Zhang C, Liu H, Yang X, et al.RRID:AB_1236466,Cat# 124208; RRID:AB_1236466 Antibody,anti-BrdU FITC (Mouse monoclonal),Biolegend,Cat# 364104; RRID:AB_2564481,Cat# 364104; RRID:AB_2564481 Antibody,anti-BrdU FITC (3D4),Biolegend,Cat# 364104;

(8) Induction of Foxp3 and activation of Tregs by HSP gp96 for treatment of autoimmune diseasesiScienceDecember 17, 2021Xu Y, Liu E, Xie X, Wang J, Zheng H, Ju Y, Chen L, Li C, Zhou X, Li Z, et al.RRID:AB_313494 CD45.2-PerCP/Cy5.5 (104),Biolegend,Cat#109827; RRID:AB_893352 BrdU-FITC (3D4),Biolegend,Cat#364103; RRID:AB_2564480 "Phospho-IkB alpha (Ser32, Ser36)",eBioscience,Cat#50-9035-42; RRID:AB_2574311 PD-1-FITC

(9) ATR inhibition reverses the resistance of homologous recombination deficient MGMTlow/MMRproficient cancer cells to temozolomideOncotargetOctober 12, 2021Lara H. El Touny et al.with 0.1 M sodium borate, pH 8.5. Cells were then incubated with anti-BrdU FITC (Biolegend, Clone 3D4, 364104) (5 μl in 100 μl cells suspended in 0.2% BSA in PBS) for 20 min at RT, spun down then resuspended in

(10) TAZ maintains telomere length in TNBC cells by mediating Rad51C expressionBreast cancer research : BCRSeptember 6, 2021Yang L, Wang B, Jiao X, Zhou C, Chen S, Gao X, Sun W, Song S, Li J, Liu J, et al.Cells were fixed in 70% ethanol and incubated with 2N HCl and 0.1M Na2B4O7. Anti-BrdU antibodies (#364103, BioLegend) were then added and incubated for 20 min at room temperature. FACS analysis was performed

(11) Hypersensitivity response has negligible impact on Hematopoietic Stem CellsStem Cell ReportsAugust 10, 2021Nir Bujanover, Roshina Thapa, Oron Goldstein, Leonid Olender, Omri Sharabi, Michael D. Milsom, Roi GazitCa+2 and Mg+2). BrdU intra-nuclear staining was by FITC-conjugated clone 3D4 (BioLegend catalog no. 364104). Transplantation HSCs were sorted as Lineage−SCA1+cKIT+CD150+Fgd5mCherry+ (LSKCD150+mC+), and 300

(12) Targeted protein degradation reveals a direct role of SPT6 in RNAPII elongation and terminationMolecular cellAugust 5, 2021Narain A, Bhandare P, Adhikari B, Backes S, Eilers M, Dölken L, Schlosser A, Erhard F, Baluapuri A, Wolf E.NA931V; RRID: AB_772210; Lot: 1708-9105 "FITC anti-BrdU, Mouse IgG1, kappa (clone 3D4)",BioLegend,Cat# 364104; RRID: AB_2564481; Lot: B264755 ,, Bacterial and virus strains,Bacterial and virus strains,Bacterial

(13) Metabolic reprogramming of terminally exhausted CD8+ T cells by IL-10 enhances anti-tumor immunityNature ImmunologyJune 1, 2021Yugang Guo et al.

(14) E47 upregulates ΔNp63α to promote growth of squamous cell carcinomaCell Death & DiseaseApril 8, 2021Jing Xu, Fengtian Li, Ya Gao, Rongtian Guo, Liangping Ding, Mengyuan Fu, Yong Yi, Hu Chen, Zhi-Xiong Jim Xiao, Mengmeng Niutreatment of DNase I at 37 °C for 1 h. Cells were then incubated with 5 μL FITC-conjugated anti-BrdU (364103, Biolegend, San Diego, CA, USA) or isotype control (400137, Biolegend, San Diego, CA, USA) antibody

(15) Chaperone-mediated autophagy sustains haematopoietic stem-cell functionNatureMarch 1, 2021Dong S, Wang Q, Kao YR, Diaz A, Tasset I, Kaushik S, Thiruthuvanathan V, Zintiridou A, Nieves E, Dzieciatkowska M, et al.were fixed, permeabilized, treated with DNase, and stained with anti-BrdU FITC antibody (Biolegend, 364103) following the BrdU labeling kit protocol (BD Pharmingen). Cells were then resuspended in staining buffer

(16) Ubiquitylation of MYC couples transcription elongation with double-strand break repair at active promotersMolecular cellFebruary 18, 2021Endres T, Solvie D, Heidelberger JB, Andrioletti V, Baluapuri A, Ade CP, Muhar M, Eilers U, Vos SM, Cramer P, et al.,Cell Signaling Technology,Cat#12265S Lot:1 RRID: AB_2797862 Mouse monoclonal FITC anti-BrdU (clone 3D4),Biozol / BioLegend,Cat#364104 RRID: AB_2564481 Donkey polyclonal anti-goat IgG-HRP secondary antibody,Santa Cruz Biotechnology,Cat#sc-2020

(17) Dual Role of Interleukin-10 in Murine NZB/W F1 LupusInternational journal of molecular sciencesJanuary 29, 2021Amend A, Wickli N, Schäfer AL, Sprenger DTL, Manz RA, Voll RE, Chevalier N.(109227, BioLegend), IgG1 FITC (553443, BD Biosciences), IgG3 FITC (553403, BD Biosciences), BrdU FITC (364103, BioLegend), NK1.1 PE (108707, BioLegend), ICOSL PE (107405, BioLegend), FoxP3 PE (12577382, eBioscience),

(18) Repurposing the Antidepressant Sertraline as SHMT Inhibitor to Suppress Serine/Glycine Synthesis–Addicted Breast Tumor GrowthMolecular Cancer TherapeuticsJanuary 1, 2021Shauni Lien Geeraerts et al.fixation, 2 M HCl denaturation, 0.5 M EDTA (pH 8.0) neutralization, BrdU-FITC antibody (BioLegend #364104, RRID:AB_2564481) staining for 20 minutes at RT and 20 minutes incubation with PI solution (100 μg/ml)

(19) A Cdh1-FoxM1-Apc axis controls muscle development and regenerationCell death & diseaseMarch 9, 2020Chen Z, Li L, Xu S, Liu Z, Zhou C, Li Z, Liu Y, Wu W, Huang Y, Kuang M, et al.min, and blocked with 2% horse serum for 1 h. Then cells were stained with anti-BrdU-FITC (Biolegend, 364104) for 30 min and analyzed by flow cytometry. Immuofluorescence Myofibers or SCs were fixed with 4%

(20) Increased Neural Progenitor Proliferation in a hiPSC Model of Autism Induces Replication Stress-Associated Genome InstabilityCell stem cellFebruary 6, 2020Wang M, Wei PC, Lim CK, Gallina IS, Marshall S, Marchetto MC, Alt FW, Gage FH.permeabilized with 0.1% Triton X-100, and treated with 2N HCl for 30 min prior to BrdU antibody (BioLegend, 364104) labeling. Cells were washed with PBS and treated with 20 μg/ml RNase A (Thermo Fisher Scientific) and

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FITC anti-BrdU from BioLegend

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FITC anti-BrdU from BioLegend

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